Excited to finally get our hands on a production Topo kit! @articnetwork.bsky.social

We’re continuing to expand the number of pathogens we support for Freyja (github.com/andersen-lab...), and developing corresponding low-cost and highly sensitive amplicon sequencing schemes as our Modjadji initiative (including @scalene.bsky.social ‬ and @chris.primalscheme.com

Although SMART-9N was developed for RNA viruses, DNA pathogens can be detect either via transcripts or random amplification of genomic DNA. This is the basis of the viral arm of the @nanoporetech.com rapid metagenomic sequencing protocol (nanoporetech.com/document/rap...)

And don't forget to watch the accompanying video of @scalene.bsky.social broodily walking the canals of Birmingham describing how sequencing can be useful in outbreaks: www.youtube.com/watch?embeds...

Working a treat here to, threw in some melt in nut inserts etc 😊

Looking forward to that!

Oh and Josh, I just wanted to let you know that I refer to your excellent 'lab-in-a-suitcase' work as a primary example of situations where the DIYNAFLUOR may find utility. It typically shows up in the first 1 or 2 slides of any presentation I make. 😅

For ONT libraries being able to measure in the range 1-100ng/ul is perfectly adequate.

A colleague here and I had an idea to use a dye with a larger Stokes shift which might help with some of the scatter but I honestly don’t think it’s necessary now.

I had been wanting to do this for a while. I got as far as dismantling one and measuring the spectra of the filter set.

This will be huge for LIAS because the fluorometer was one of the most expensive bits. Qubit 4 currently £2800 on our procurement system!