Bocobza lab @volcani
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Bocobza lab @volcani
@sbocobza.bsky.social
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Lab of Samuel Bocobza, at the Volcani Center, dedicated to tissue culture and genome editing.
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Reposted by Bocobza lab @volcani
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📅New #PlantSciEvents Event Added: Plant Tissue Culture and Genome Engineering - 2026

👉https://buff.ly/48E3mS5

#PlantScience
Plant Tissue Culture and Genome Engineering - 2026
(2026) Sun 10 May - Wed 13 [EDT]: We are excited to announce that the International Conference on Plant Tissue Culture & Genome Engineering (PTCGE 2026) will take place from May 10–13, 2026, in Santa…
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October 24, 2025 at 2:15 PM
Reposted by Bocobza lab @volcani
jxbotany.bsky.social
🫑 TECHNICAL INNOVATION 🫑

Breaking the glass ceiling of stable genetic transformation and gene editing in the popular pepper cv Cayenne - Kumar et al.

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪
Genetic transformation of pepper plants using an anthocyanin-based visual marker and a silencing suppressor. (A) Schematic representation of the T-DNAs built to overexpress CaAN2 (CaAN2-OE) and to silence CaSGS3 (CaAN2-OE_CaSGS3i) S, sense; AS, antisense. (B) Shoots developing in tissue culture 8 weeks after transformation. Scale bar: 0.4 cm. (C) Pictures of plants, flowers, and fruits obtained after transformation. Wild-type plants were not transformed and are shown as negative control. (D) Rate of purple or green shoots and PCR-positive shoots obtained after transformation with the indicated constructs. (E) Analysis of the relative transcript levels of CaSGS3 and CaAN2 using qRT-PCR. The data represent the mean ±SD from three biological replicates (n=3); Student’s t-test: *P<0.05; **P<0.01.
July 16, 2025 at 3:08 PM