If you would like more information or would like to collaborate with our teams to obtain pea mutants, please do not hesitate to contact us.

This protocol was developed thanks to the TYPEX project, as part of the PEPR - advanced varietal selection (SVA) by the SAS and Dragon teams of the IJPB @ijpb-versaillescly.bsky.social of the INRAE BAP department.

3/3 The protocol produces edited plants as early as the T0 transformed generation, and stable, non-transgenic lines at T1 generation.

2/3 The use of a fluorescent marker enabled rapid, non-destructive detection of transformed shoots. Combining this approach with a grafting strategy overcame the difficulties of root regeneration, a major obstacle in pea.

1/3This innovative protocol, based on the use of the CRISPR-Cas9 system, enables edited plants to be obtained rapidly. The protocol is based on the editing of the TENDRIL-LESS gene, the mutation of which generates a phenotype that can be easily observed by the transformation of tendrils intoleaflets