🔬This paper explores the role of the primary cilium in neuronal migration. Using live-imaging, this study demonstrates the mechanosensory function of GPR161 receptor and its role on the cAMP/PKA signaling pathway, which in turn regulates the organization of the microtubule cage around the nucleus.

Our work investigates the dynamics of muscle stem cells in DMD pathology. We demonstrate stem cell migration impairment (fibre dependent) and premature differentiation driven by unbalanced symmetric divisions (fibre independent, regulated by Pi3K/Akt and p38)🎥💪🐭

Static methods have reported conflicting results about dystrophic stem cells. But actually capturing their temporal dynamics is really key to understand critical aspects of the disease!

👏👏 Amazing work @sardeliza.bsky.social, @gaellel.bsky.social , H. Varet, V. Laville, J. Fernandes, @tajbakhshlab.bsky.social

@devstempasteur.bsky.social @pasteur.fr @cnrsbiologie.bsky.social

Key findings 👇

Using in vivo live imaging, we showed that dystrophic stem cells have impaired migration kinetics and precocious differentiation.

We developed an ex vivo live imaging assay with stem cells within their muscle fibre niche to recapitulate the in vivo phenotypes and allow for perturbation studies.

Ex vivo dystrophic stem cells indeed showed altered migration and precocious differentiation, through imbalanced symmetric divisions.

To explore the contribution of autonomous vs niche-driven cues, we set up a niche switching assay between healthy and dystrophic stem cells and muscle fibres.

(YFP-labelled dystrophic stem cell grafted to a healthy fibre here)

Stem cell fate decisions were cell-autonomous, while their migration behaviour was largely determined by the healthy vs dystrophic muscle fibre niches.

For mechanistic studies we developed an in vitro live imaging assay with isolated stem cells.

The differentiation of dystrophic stem cells relied on dual p38–PI3K signalling, an unrecognised synergy not seen in healthy cells which depended solely on p38.

Our findings set Duchenne Muscular Dystrophy also as a stem cell disease, prior to the well-established muscle fibre fragility.
Hope you'll like it, feedback much appreciated!

Excited to share that the preprint of my Ph.D. work is now available! 🎉 Huge thanks to my amazing co-authors for their support and collaboration: @gaellel.bsky.social, H. Varet, V. Laville, J. Fernandes, @tajbakhshlab.bsky.social, @brendan-evano.bsky.social.
@devstempasteur.bsky.social @pasteur.fr