Sam Manna
@sammanna.bsky.social
Microbiologist/postdoc working on #pneumococcus. Former Lecturer. Ex-mitochondrial geneticist. Vice President of Communications for @aussocmic.bsky.social Views are my own.
Together, we provide the requisite genetic, serological and biochemical evidence to support 33G-like be designated as a new serotype, which we have named 33H. So far, we have seen this new serotype in Mongolia and South Africa - very excited to see where else it might be!
March 25, 2025 at 7:29 AM
Together, we provide the requisite genetic, serological and biochemical evidence to support 33G-like be designated as a new serotype, which we have named 33H. So far, we have seen this new serotype in Mongolia and South Africa - very excited to see where else it might be!
Lastly, to confirm the lack of acetylation in the 33G-like capsule due to the loss of wciG, we conducted 1H NMR on the capsular polysaccharide, which showed that in contrast with 33G capsule, the 33G-like capsule lacks any acetyl residues.
March 25, 2025 at 7:29 AM
Lastly, to confirm the lack of acetylation in the 33G-like capsule due to the loss of wciG, we conducted 1H NMR on the capsular polysaccharide, which showed that in contrast with 33G capsule, the 33G-like capsule lacks any acetyl residues.
When we examined the capsule locus, the 33G-like capsule locus was identical to that of 33G except for one difference - all the 33G-like isolates had frameshift mutations in a capsule gene called wciG, which encodes an acetyltransferase.
March 25, 2025 at 7:29 AM
When we examined the capsule locus, the 33G-like capsule locus was identical to that of 33G except for one difference - all the 33G-like isolates had frameshift mutations in a capsule gene called wciG, which encodes an acetyltransferase.