and the residue from the charge making it through to the sample. Had to shoot through screen to keep things relatively clean.

I assume the patent on this must have expired by now. I was an academic when I was actively shooting so less concerned about it. The helium gun was so much better than the guns using gun powder blanks to accelerate the carrier because of the variability of the charges …

Cut syringes to use as forms for plating suspension culture onto whatman paper back in the day. About half a day mucking around figuring out the right amount of pre-wetting necessary so the cells spread uniformly. And even then the first experiment was 🤞

It is satisfying once you get in the groove. Less satisfying for people unfamiliar with the tech because they expect more. Unfortunately, when it gets exciting it usually means you have issues and all that prep work goes bye-bye.

Frozen away in single use aliquots as cold as you can manage.

Always fun to prep, but not the worst.

And that’s one of the big takeaways from all this. People tolerate a$$holes for reasons, instead of removing them from the system.

The place I did my graduate work at had a mix of exceptional scientists and mentors as well as abusive mentors that were tolerated.

How do you read this graph? Two y-axes, no labels, and different ranges on each y-axis without any clue as to which line corresponds to which y-axis.

Not blaming you as it isn’t your creation (I assume), just trying to understand what you’re posting.

It was a recombination meeting in the late 80s or early 90s. I was working on FLP recombinase at the time. The highlight of the conference for me was getting to talk briefly with Frank Stahl. Working for M. Jayaram at the time. We were just figuring out the mechanism of the rxn.

Just an absolute banger of an animal family

But you do have a bunch of wild-type to compare it to so I will try to be patient

Does it have the normal number of sepals?

As someone who routinely eats his apple pie topped with a slice of cheddar cheese, I just sort of shrug at this. Probably wouldn’t buy it for myself, but probably would try it if offered.

A membership to your local zoo/aquarium/kid-friendly museum pays for itself when you leave “early” for reasons. Reduces everyone’s stress during the visit because you can always pop back in at a later date. And if your child develops a favorite animal, allows otter trips.

I have seen occasional preps of 10% Triton grow a pet, but never seen it in a stock bottle.

Maybe just good sterile technique at the bench over working in a hood?

But my strategy is based on doing plant media in a hood and bacterial media at the bench.

The screen has to be fairly thick to effectively stop the rupture disk flat. If it deforms, it alters the spray pattern.