Rohit V. Pappu
@rohitpappu68.bsky.social
Biophysics, soft matter physics, intrinsically disordered proteins, macromolecular interactions, & phase transitions are my interests. Discovery through rigor and collaboration is my passion. Kindness, generosity, humility, and justice are my hopes.
Reposted by Rohit V. Pappu
Now in print: our study using single #fluorogenic molecules to probe the network architecture within #biomolecular #condensates with @rohitpappu68.bsky.social @washuengineers.bsky.social . Teaser video below. Read the full article at doi.org/10.1038/s415... #singlemolecule
May 30, 2025 at 4:16 AM
Now in print: our study using single #fluorogenic molecules to probe the network architecture within #biomolecular #condensates with @rohitpappu68.bsky.social @washuengineers.bsky.social . Teaser video below. Read the full article at doi.org/10.1038/s415... #singlemolecule
Do read the result of this collaboration that dissected the reaction from all sides, even showing how beautifully the results transfer over into cells, where hnRNP-A1 with ALS-associated mutations can be redesigned to generate wild-type-like stress granule phenotypes through tunable metastability.
May 28, 2025 at 3:42 PM
Do read the result of this collaboration that dissected the reaction from all sides, even showing how beautifully the results transfer over into cells, where hnRNP-A1 with ALS-associated mutations can be redesigned to generate wild-type-like stress granule phenotypes through tunable metastability.
Using kinetic models inspired by Tuomas Knowles, we predicted that efflux of material from metastable condensates becomes rate-limiting for fibril formation. Efflux slows as metastability increases. Experiments with @priya-r-banerjee.bsky.social confirmed these predictions.
May 28, 2025 at 3:42 PM
Using kinetic models inspired by Tuomas Knowles, we predicted that efflux of material from metastable condensates becomes rate-limiting for fibril formation. Efflux slows as metastability increases. Experiments with @priya-r-banerjee.bsky.social confirmed these predictions.
The Ostwald rule of stages is central to thinking about how stable vs. metastable states contribute to nucleation & growth of fibrils. This important linkage between thermodynamics & kinetics is often glossed over generating confusion about the role of condensates in fibril formation.
May 28, 2025 at 3:42 PM
The Ostwald rule of stages is central to thinking about how stable vs. metastable states contribute to nucleation & growth of fibrils. This important linkage between thermodynamics & kinetics is often glossed over generating confusion about the role of condensates in fibril formation.
Relative stabilities of condensates vs. fibrils turned out to be crucial for guiding mechanistic studies. Measurements of relative stabilities showed that condensates are metastable. Condensate interiors ≠ crucibles & the sink potentials of condensates are tunable via tunable metastability.
May 28, 2025 at 3:42 PM
Relative stabilities of condensates vs. fibrils turned out to be crucial for guiding mechanistic studies. Measurements of relative stabilities showed that condensates are metastable. Condensate interiors ≠ crucibles & the sink potentials of condensates are tunable via tunable metastability.
The Center for Biomolecular Condensates - making things happen!
April 11, 2025 at 6:16 PM
The Center for Biomolecular Condensates - making things happen!
This work helps uncover rules regarding organization at condensate interfaces while also showing how systems with interfacial preferences can be designed & measurements can help extract the organization at interfaces. Collaborations with @lewlab.com, Knowles lab, driven by Nadia Erkamp & Mina Farag
April 11, 2025 at 3:23 PM
This work helps uncover rules regarding organization at condensate interfaces while also showing how systems with interfacial preferences can be designed & measurements can help extract the organization at interfaces. Collaborations with @lewlab.com, Knowles lab, driven by Nadia Erkamp & Mina Farag
Computations show that adsorbents prefer more parallel orientations whereas scaffolds prefer more perpendicular orientations at interfaces. Unprecedented single fluorogen tracking quantifies dynamical anisotropy at interfaces with faster motions parallel to interfaces.
April 11, 2025 at 3:23 PM
Computations show that adsorbents prefer more parallel orientations whereas scaffolds prefer more perpendicular orientations at interfaces. Unprecedented single fluorogen tracking quantifies dynamical anisotropy at interfaces with faster motions parallel to interfaces.