REMOA-Spanish Network for Advanced Microscopy
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REMOA promotes knowledge transfer and collaboration between bioimaging microscopists, developers, scientists and analysts
Organoid Oasis
ALI (Air–Liquid Interface) is a strategy for generating organoids from minced tissue, offering the advantage of preserving both tissue parenchyma and stroma.
Epithelial cells: EpCAM (green,AF555)
Immune cells: CD45 (cyan,FITC)
Fibroblasts: α-SMA (magenta,AF647)
Nuclei: DAPI (blue)
ALI (Air–Liquid Interface) is a strategy for generating organoids from minced tissue, offering the advantage of preserving both tissue parenchyma and stroma.
Epithelial cells: EpCAM (green,AF555)
Immune cells: CD45 (cyan,FITC)
Fibroblasts: α-SMA (magenta,AF647)
Nuclei: DAPI (blue)
October 23, 2025 at 8:10 PM
Organoid Oasis
ALI (Air–Liquid Interface) is a strategy for generating organoids from minced tissue, offering the advantage of preserving both tissue parenchyma and stroma.
Epithelial cells: EpCAM (green,AF555)
Immune cells: CD45 (cyan,FITC)
Fibroblasts: α-SMA (magenta,AF647)
Nuclei: DAPI (blue)
ALI (Air–Liquid Interface) is a strategy for generating organoids from minced tissue, offering the advantage of preserving both tissue parenchyma and stroma.
Epithelial cells: EpCAM (green,AF555)
Immune cells: CD45 (cyan,FITC)
Fibroblasts: α-SMA (magenta,AF647)
Nuclei: DAPI (blue)
🧠✨
Neural stem cells were cultured adherent on Matrigel and then fixed with 4% PFA. The samples were stained with DAPI and TOM20.
After staining, a 4× expansion microscopy protocol was applied. Samples were imaged using a Zeiss LSM980 Airyscan confocal microscope with a 40× water-immersion obj.
Neural stem cells were cultured adherent on Matrigel and then fixed with 4% PFA. The samples were stained with DAPI and TOM20.
After staining, a 4× expansion microscopy protocol was applied. Samples were imaged using a Zeiss LSM980 Airyscan confocal microscope with a 40× water-immersion obj.
October 23, 2025 at 8:06 PM
🧠✨
Neural stem cells were cultured adherent on Matrigel and then fixed with 4% PFA. The samples were stained with DAPI and TOM20.
After staining, a 4× expansion microscopy protocol was applied. Samples were imaged using a Zeiss LSM980 Airyscan confocal microscope with a 40× water-immersion obj.
Neural stem cells were cultured adherent on Matrigel and then fixed with 4% PFA. The samples were stained with DAPI and TOM20.
After staining, a 4× expansion microscopy protocol was applied. Samples were imaged using a Zeiss LSM980 Airyscan confocal microscope with a 40× water-immersion obj.
VGlut2 marks thalamic axon terminals whose projections onto the cortex form circular structures “barrels”, characteristic of somatosensory cortex.
Syt2 labels axon terminals of parvalbumin neurons, corresponding to inhibitory synapses that play crucial role in maintaining balanced neuronal activity.
Syt2 labels axon terminals of parvalbumin neurons, corresponding to inhibitory synapses that play crucial role in maintaining balanced neuronal activity.
October 23, 2025 at 8:01 PM
VGlut2 marks thalamic axon terminals whose projections onto the cortex form circular structures “barrels”, characteristic of somatosensory cortex.
Syt2 labels axon terminals of parvalbumin neurons, corresponding to inhibitory synapses that play crucial role in maintaining balanced neuronal activity.
Syt2 labels axon terminals of parvalbumin neurons, corresponding to inhibitory synapses that play crucial role in maintaining balanced neuronal activity.
🧠🌈 Developing Connectivity in the Mouse Brain
Parvalbumin marks a small subset of cortical interneurons (≈10%) essential for proper brain development and closely linked to neurodevelopmental disorders.
Parvalbumin marks a small subset of cortical interneurons (≈10%) essential for proper brain development and closely linked to neurodevelopmental disorders.
October 23, 2025 at 8:01 PM
🧠🌈 Developing Connectivity in the Mouse Brain
Parvalbumin marks a small subset of cortical interneurons (≈10%) essential for proper brain development and closely linked to neurodevelopmental disorders.
Parvalbumin marks a small subset of cortical interneurons (≈10%) essential for proper brain development and closely linked to neurodevelopmental disorders.
Tittle: Fan-nomenal Structure
Imaged using ZEISS LS Z.1 with a 20x objective (NA 1.0, WD 1.8 mm, RI 1.33; W Plan-Apochromat). The Coral was cleared using 2ECi and stained with Propidium iodide. Final image consists of multiple tile stitching, deconvolution and 3D rendering (ZEISS Arivis Pro 4.3.0).
Imaged using ZEISS LS Z.1 with a 20x objective (NA 1.0, WD 1.8 mm, RI 1.33; W Plan-Apochromat). The Coral was cleared using 2ECi and stained with Propidium iodide. Final image consists of multiple tile stitching, deconvolution and 3D rendering (ZEISS Arivis Pro 4.3.0).
October 23, 2025 at 7:42 PM
Tittle: Fan-nomenal Structure
Imaged using ZEISS LS Z.1 with a 20x objective (NA 1.0, WD 1.8 mm, RI 1.33; W Plan-Apochromat). The Coral was cleared using 2ECi and stained with Propidium iodide. Final image consists of multiple tile stitching, deconvolution and 3D rendering (ZEISS Arivis Pro 4.3.0).
Imaged using ZEISS LS Z.1 with a 20x objective (NA 1.0, WD 1.8 mm, RI 1.33; W Plan-Apochromat). The Coral was cleared using 2ECi and stained with Propidium iodide. Final image consists of multiple tile stitching, deconvolution and 3D rendering (ZEISS Arivis Pro 4.3.0).