Mark Rebeiz
@rebeizlab.bsky.social
Laboratory of Mark Rebeiz (he/they), studying the evolution and development of morphological novelty in Drosophila at the University of Pittsburgh. Rooting for team human.
https://rebeizlab.weebly.com/
https://rebeizlab.weebly.com/
Thanks so much Amber! Hope you are having an awesome time in your postdoc!
October 30, 2024 at 6:06 PM
Thanks so much Amber! Hope you are having an awesome time in your postdoc!
BTW our bad - Gavin's handle is @gavinrrice.bsky.social
October 28, 2024 at 7:33 PM
BTW our bad - Gavin's handle is @gavinrrice.bsky.social
Thank you to the O'Connor-Giles lab for updating the incredible CRISPR Optimal Target Finder to include D. eugracilis. @bdsc.bsky.social for lines, and @flybase.bsky.social for being our favorite database
October 28, 2024 at 7:32 PM
Thank you to the O'Connor-Giles lab for updating the incredible CRISPR Optimal Target Finder to include D. eugracilis. @bdsc.bsky.social for lines, and @flybase.bsky.social for being our favorite database
We want to thank our amazing collaborators Julia Zeitlinger and Tatiana Gaitan. We cannot wait to share more as we continue to dive deeper into how the trichome genetic network has specialized!
October 28, 2024 at 7:30 PM
We want to thank our amazing collaborators Julia Zeitlinger and Tatiana Gaitan. We cannot wait to share more as we continue to dive deeper into how the trichome genetic network has specialized!
Our results demonstrate the power of genetic network co-option to redeploy a genetic network into a new tissue context. Yet, novelties are more than mere iterations of repeated structures. We reflect on how they derive unique features by elaborating their network architecture.
October 28, 2024 at 7:29 PM
Our results demonstrate the power of genetic network co-option to redeploy a genetic network into a new tissue context. Yet, novelties are more than mere iterations of repeated structures. We reflect on how they derive unique features by elaborating their network architecture.
Misexpression of the active form of shavenbaby in the naïve aedeagal sheath of D. melanogaster was sufficient to induce small trichomes.
This is one of the first times that genetic network co-option has been recapitulated through experimental manipulations.
This is one of the first times that genetic network co-option has been recapitulated through experimental manipulations.
October 28, 2024 at 7:27 PM
Misexpression of the active form of shavenbaby in the naïve aedeagal sheath of D. melanogaster was sufficient to induce small trichomes.
This is one of the first times that genetic network co-option has been recapitulated through experimental manipulations.
This is one of the first times that genetic network co-option has been recapitulated through experimental manipulations.
Disruption of shavenbaby via mosaic CRISPR caused the truncation of trichome height, showing the important role of shavenbaby, but also indicating that there are likely other redundant transcription factors as well
October 28, 2024 at 7:26 PM
Disruption of shavenbaby via mosaic CRISPR caused the truncation of trichome height, showing the important role of shavenbaby, but also indicating that there are likely other redundant transcription factors as well
Additionally, we find that the trichome genetic network was partially co-opted into the aedeagal sheath as 14 (purple) out of 23 direct targets of shavenbaby we tested have gained expression in the D. eugracilis aedeagal sheath
October 28, 2024 at 7:26 PM
Additionally, we find that the trichome genetic network was partially co-opted into the aedeagal sheath as 14 (purple) out of 23 direct targets of shavenbaby we tested have gained expression in the D. eugracilis aedeagal sheath
The novel unicellular outgrowths of D. eugracilis have evolved expression of the key trichome transcription factor shavenbaby, named for its mutant phenotype, in which trichome morphology is disrupted
October 28, 2024 at 7:25 PM
The novel unicellular outgrowths of D. eugracilis have evolved expression of the key trichome transcription factor shavenbaby, named for its mutant phenotype, in which trichome morphology is disrupted
We found that each of these outgrowths develop as an actin-rich (magenta) extension of a single cell (apical cell junctions grey), similar to trichomes, hair-like structures that cover the larvae and much of the adult cuticle
October 28, 2024 at 7:24 PM
We found that each of these outgrowths develop as an actin-rich (magenta) extension of a single cell (apical cell junctions grey), similar to trichomes, hair-like structures that cover the larvae and much of the adult cuticle
We developed the rapidly evolving drosophilid terminalia as a model to explore the genetic basis of novelty. D. eugracilis has over 150 differently sized outgrowths on its aedeagal sheath which appear to be absent from other species of Drosophila, including D. melanogaster.
October 28, 2024 at 7:23 PM
We developed the rapidly evolving drosophilid terminalia as a model to explore the genetic basis of novelty. D. eugracilis has over 150 differently sized outgrowths on its aedeagal sheath which appear to be absent from other species of Drosophila, including D. melanogaster.