Rajiv K. Khajuria
@raj-khaj.bsky.social
Reposted by Rajiv K. Khajuria
Distinct tumor immune microenvironmental (TIME) landscapes drive divergent immunotherapy responses in glioblastoma https://www.biorxiv.org/content/10.1101/2025.10.18.683203v1
October 20, 2025 at 3:48 AM
Distinct tumor immune microenvironmental (TIME) landscapes drive divergent immunotherapy responses in glioblastoma https://www.biorxiv.org/content/10.1101/2025.10.18.683203v1
Reposted by Rajiv K. Khajuria
How to disconnect therapy-resistant tumor networks in glioblastoma?
In our paper, online @Cancer Discovery, we built an anti-tumor microtube (TM) screening pipeline and found that Protein Kinase C activators disrupt TMs, silence Ca²⁺ “pacemakers” and boost radiotherapy.
doi.org/10.1158/2159...
In our paper, online @Cancer Discovery, we built an anti-tumor microtube (TM) screening pipeline and found that Protein Kinase C activators disrupt TMs, silence Ca²⁺ “pacemakers” and boost radiotherapy.
doi.org/10.1158/2159...
October 9, 2025 at 8:52 PM
How to disconnect therapy-resistant tumor networks in glioblastoma?
In our paper, online @Cancer Discovery, we built an anti-tumor microtube (TM) screening pipeline and found that Protein Kinase C activators disrupt TMs, silence Ca²⁺ “pacemakers” and boost radiotherapy.
doi.org/10.1158/2159...
In our paper, online @Cancer Discovery, we built an anti-tumor microtube (TM) screening pipeline and found that Protein Kinase C activators disrupt TMs, silence Ca²⁺ “pacemakers” and boost radiotherapy.
doi.org/10.1158/2159...
TET CpG sequence context specific DNA demeth-ylation shapes progression of IDH-mutant gliomas
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
TET CpG sequence context specific DNA demeth-ylation shapes progression of IDH-mutant gliomas
Background: Treatment decisions in IDH-mutant oligodendrogliomas are shaped by tumor aggressiveness, underscoring the need for objective grading of these malignant brain tumors. Material and Methods: ...
www.biorxiv.org
October 11, 2025 at 11:56 AM
TET CpG sequence context specific DNA demeth-ylation shapes progression of IDH-mutant gliomas
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Rajiv K. Khajuria
🚨 new glioblastoma preprint alert!
we present the first spatially resolved single cell atlas comparing radionecrotic changes (RN) and recurrent IDH-wildtype glioblastoma (GB) –– shedding light on a long-standing diagnostic challenge.
🧵 1/
we present the first spatially resolved single cell atlas comparing radionecrotic changes (RN) and recurrent IDH-wildtype glioblastoma (GB) –– shedding light on a long-standing diagnostic challenge.
🧵 1/
October 6, 2025 at 12:41 PM
🚨 new glioblastoma preprint alert!
we present the first spatially resolved single cell atlas comparing radionecrotic changes (RN) and recurrent IDH-wildtype glioblastoma (GB) –– shedding light on a long-standing diagnostic challenge.
🧵 1/
we present the first spatially resolved single cell atlas comparing radionecrotic changes (RN) and recurrent IDH-wildtype glioblastoma (GB) –– shedding light on a long-standing diagnostic challenge.
🧵 1/
Genomic landscape and immunological profile of glioblastoma in East Asian patients
www.cell.com/cell-reports...
www.cell.com/cell-reports...
Genomic landscape and immunological profile of glioblastoma in East Asian patients
Zhong et al. analyze the largest East Asian glioblastoma (EAS-GBM) cohort, identifying
four transcriptomic subtypes and distinct genomic features compared to European cases.
Notably, EAS-GBMs lack EGF...
www.cell.com
September 26, 2025 at 7:02 PM
Genomic landscape and immunological profile of glioblastoma in East Asian patients
www.cell.com/cell-reports...
www.cell.com/cell-reports...
Reposted by Rajiv K. Khajuria
🔥 Our second preprint, led by Josch, introduces a new framework to measure mtDNA mutational burden & constraint at single-cell resolution.
We present:
🔹 scmtMPM (mutations per Mb)
🔹 scwMSS (heteroplasmy-weighted local constraint) 👉 www.researchsquare.com/article/rs-6...
🔹 scmtMPM (mutations per Mb)
🔹 scwMSS (heteroplasmy-weighted local constraint) 👉 www.researchsquare.com/article/rs-6...
Single-cell multi-omics resolved analysis of mitochondrial genome-wide mutational burden, constraint, and mosaicism
Mitochondria contain their own circular, multi-copy genome, encoding essential components of the mitochondrial respiratory chain vital for cellular metabolism. Mitochondrial DNA (mtDNA) mutations occu...
www.researchsquare.com
September 24, 2025 at 12:39 PM
🔥 Our second preprint, led by Josch, introduces a new framework to measure mtDNA mutational burden & constraint at single-cell resolution.
We present:
🔹 scmtMPM (mutations per Mb)
🔹 scwMSS (heteroplasmy-weighted local constraint) 👉 www.researchsquare.com/article/rs-6...
🔹 scmtMPM (mutations per Mb)
🔹 scwMSS (heteroplasmy-weighted local constraint) 👉 www.researchsquare.com/article/rs-6...
Reposted by Rajiv K. Khajuria
🚨 New preprints from our lab!
First, we introduce Cryo-mtscATAC-seq, led by Maren (@ms-maren.bsky.social ), enabling high-throughput clonal tracing from frozen human samples by isolating nuclei with their mitochondria (“CryoCells”).
👉 www.biorxiv.org/content/10.1...
Cryo-mtscATAC-seq for single-cell mitochondrial DNA genotyping and clonal tracing in archived human tissues
High-throughput clonal tracing of primary human samples relies on naturally occurring barcodes, such as somatic mitochondrial DNA (mtDNA) mutations detected via single-cell ATAC-seq (mtscATAC-seq). Fr...
www.biorxiv.org
September 24, 2025 at 12:39 PM
🚨 New preprints from our lab!
First, we introduce Cryo-mtscATAC-seq, led by Maren (@ms-maren.bsky.social ), enabling high-throughput clonal tracing from frozen human samples by isolating nuclei with their mitochondria (“CryoCells”).
👉 www.biorxiv.org/content/10.1...
Gliomas phenocopy an inborn error of metabolism to drive neuronal activity and tumor growth
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Gliomas phenocopy an inborn error of metabolism to drive neuronal activity and tumor growth
The metabolic hallmarks of high-grade glioma (HGG) are not fully understood. Human brain tissue metabolomics revealed that the creatine synthesis pathway intermediate guanidinoacetate (GAA) accumulate...
www.biorxiv.org
September 21, 2025 at 3:15 PM
Gliomas phenocopy an inborn error of metabolism to drive neuronal activity and tumor growth
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Gain-of-function mutant p53 regulates long-noncoding RNA LINC00643 to modulate HIF1α in glioblastoma
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Gain-of-function mutant p53 regulates long-noncoding RNA LINC00643 to modulate HIF1α in glioblastoma
Gain-of-function mutations of p53 (GOF-MUT-p53) act as oncogenes by regulating gene transcription. We screened for the genome-wide transcriptional targets of GOF-MUT-p53 in glioblastoma (GBM) and foun...
www.biorxiv.org
September 21, 2025 at 3:13 PM
Gain-of-function mutant p53 regulates long-noncoding RNA LINC00643 to modulate HIF1α in glioblastoma
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Restraint of cancer cell plasticity by spatial homotypic clustering: Cancer Cell
www.cell.com/cancer-cell/...
www.cell.com/cancer-cell/...
Restraint of cancer cell plasticity by spatial homotypic clustering
Migliozzi et al. use single-cell resolution spatial transcriptomics of 16 glioblastoma
and 2.8 million cells to study tumor architecture. They discover that cell state coherence
is maximal in cells or...
www.cell.com
September 20, 2025 at 8:12 PM
Restraint of cancer cell plasticity by spatial homotypic clustering: Cancer Cell
www.cell.com/cancer-cell/...
www.cell.com/cancer-cell/...
Integrative multi-omics combined with functional pharmacological profiling in patient-derived organoids identifies personalized therapeutic vulnerabilities of adult high-grade gliomas
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Integrative multi-omics combined with functional pharmacological profiling in patient-derived organoids identifies personalized therapeutic vulnerabilities of adult high-grade gliomas
Background: Precision medicine has transformed cancer treatment by tailoring therapies to specific molecular aberrations. Integrating high-resolution multi-omics with high-throughput functional profil...
www.biorxiv.org
September 15, 2025 at 10:23 PM
Integrative multi-omics combined with functional pharmacological profiling in patient-derived organoids identifies personalized therapeutic vulnerabilities of adult high-grade gliomas
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Protein-based classification reveals an immune-hot subtype in IDH mutant astrocytoma with worse prognosis
www.cell.com/cancer-cell/...
www.cell.com/cancer-cell/...
Protein-based classification reveals an immune-hot subtype in IDH mutant astrocytoma with worse prognosis
Tang et al. apply spatiotemporal multi-omics to define four proteomic subtypes of
IDH-mutant astrocytoma. They highlight an immune/mesenchymal-enriched (IME) subtype
marked by gemistocytic differentia...
www.cell.com
September 15, 2025 at 8:27 PM
Protein-based classification reveals an immune-hot subtype in IDH mutant astrocytoma with worse prognosis
www.cell.com/cancer-cell/...
www.cell.com/cancer-cell/...