6/

"We hypothesized that patients with high expression levels of XBP1 target genes might resist Bortezomib more effectively. Our research in clinical trials confirmed this, predicting patient survival with bortezomib—but not with other drugs, highlighting the specificity of our findings. "

5/6

"Stress can lead to cell death or survival. Chemotherapies like bortezomib induce ER stress"

We discovered a subpopulation of bortezomib-resistant cells that survive, reliant on IRE-1, raising questions about the role of IRE1 and XBP1 in patient survival under bortezomib treatment."

4/6 "Using SNUPR, we observed diverse cellular responses to the same stress: some cells block protein translation, others pause and recover, while some don’t stop at all. Not all induce XBP1 translocation—some massively do.

Discover much more about their intricate interactions in our paper! 📑"

"Western blot and qPCR are bulk and can be tedious, so we developed SNUPR"

Think of it like flow cytometry, but for nuclei. SNUPR allows to more efficiently track transcription factor translocation, and translation without cytoplasmic interference, offering clearer, more specific results."

2/6
ER stress starts with the accumulation of misfolded proteins in the ER. This results in the activation of sensors that mediate molecular responses, resulting in cell adaptation or cell death.

The interplay between sensors and resulting cellular responses are challenging to study, SO..

1/6📣 1st thread in the BlueSky! Speakers ON!

To honor his creative PhD :
A blend of videos & with lyrics based on our latest research:

Enjoy & 🙏 share!

Single-Nuclei Analysis of the Unfolded Protein Response (#SNUPR): Revealing BTZ Resistance in Multiple Myeloma

www.biorxiv.org/content/10.1...