If we looked at cell-cell adhesions we saw the exact same thing! Both Trip6 and LIMD1, tension sensitive LIM domain proteins, showed sudden large drops in intensity in response to changes in tension on the adhesion.

So we next looked at other FA proteins, including paxillin, kindlin2, and PINCH. Only paxillin, another tension sensitive LIM domain protein showed changes in response to drops in tension!

We next tried the same experiments with vinculin, a known mechanosensitive FA protein. What we found was a total surprise - while zyxin was responsive to changes in tension, vinculin accumulation was completely unchanged!

We used a laser to damage a stress fiber (SF) upstream of an adhesion and see what happened to zyxin at the adhesion. We saw both zyxin intensity and traction stresses drop immediately in response to the laser ablation.

There are some good local RhoA activation movies in one of our old papers for this pubmed.ncbi.nlm.nih.gov/28604737/

this is myosin being recruited