Nathan Ponzar
@ponzponzin.bsky.social
Postdoctoral biochemist at Saint Louis University.
Peeling back the veil of ignorance one experiment at a time.
Primary interest is in fundamental aspects of enzymology, e.g. catalysis, substrate recognition, ligand effects, etc.
Peeling back the veil of ignorance one experiment at a time.
Primary interest is in fundamental aspects of enzymology, e.g. catalysis, substrate recognition, ligand effects, etc.
(2/2)
Mass equiv:
V1C1 +V2C2=V3C3
V1=100ul, C1=8.2uM
C2=200uM, V2 is __?.
C3 =10uM, V3 is __?, but...
V3=V1 +V2
Substitute
V1C1 +V2C2 = (V1 +V2)C3
Expand
V1C1 +V2C2 = V1C3 +V2C3
Subtract
V2C2 -V2C3 = V1C1 +V1C3
Factor
V2(C2-C3) = V1(C1+C3)
Divide
V2 = V1(C1+C3)/(C2-C3)
Plug & chug
9.6 ul
Mass equiv:
V1C1 +V2C2=V3C3
V1=100ul, C1=8.2uM
C2=200uM, V2 is __?.
C3 =10uM, V3 is __?, but...
V3=V1 +V2
Substitute
V1C1 +V2C2 = (V1 +V2)C3
Expand
V1C1 +V2C2 = V1C3 +V2C3
Subtract
V2C2 -V2C3 = V1C1 +V1C3
Factor
V2(C2-C3) = V1(C1+C3)
Divide
V2 = V1(C1+C3)/(C2-C3)
Plug & chug
9.6 ul
December 26, 2024 at 11:30 PM
(2/2)
Mass equiv:
V1C1 +V2C2=V3C3
V1=100ul, C1=8.2uM
C2=200uM, V2 is __?.
C3 =10uM, V3 is __?, but...
V3=V1 +V2
Substitute
V1C1 +V2C2 = (V1 +V2)C3
Expand
V1C1 +V2C2 = V1C3 +V2C3
Subtract
V2C2 -V2C3 = V1C1 +V1C3
Factor
V2(C2-C3) = V1(C1+C3)
Divide
V2 = V1(C1+C3)/(C2-C3)
Plug & chug
9.6 ul
Mass equiv:
V1C1 +V2C2=V3C3
V1=100ul, C1=8.2uM
C2=200uM, V2 is __?.
C3 =10uM, V3 is __?, but...
V3=V1 +V2
Substitute
V1C1 +V2C2 = (V1 +V2)C3
Expand
V1C1 +V2C2 = V1C3 +V2C3
Subtract
V2C2 -V2C3 = V1C1 +V1C3
Factor
V2(C2-C3) = V1(C1+C3)
Divide
V2 = V1(C1+C3)/(C2-C3)
Plug & chug
9.6 ul