4/ Using calcium and GRAB sensor imaging in the choroid plexus epithelium, we demonstrated that CSF serotonin likely impacts choroid plexus secretion. MORSE can thus capture dynamic composition of brain fluids with sufficient sensitivity to detect biologically relevant signals.

2/ In acute brain slices, we used the probes to quantify serotonin and norepinephrine release in response to depolarization. We were also able to unmix the catecholamine crosstalk at the norepinephrine and dopamine GRAB sensors by recording both sensors at the same time.

I’m thrilled to share our preprint presenting MORSE: a new way to simultaneously & quantitatively track patterns of neuromodulators/neuropeptides in vitro & in vivo using spatially multiplexed 3D imaging of 10+ green optical sensors on a microendoscope
biorxiv.org/content/10.1101/2025.01.26.634931v1