MHC peptide predictions are accurate but fail to identify the few immunodominant epitopes within the larger list of binders.

There many epitopes that could induce a strong T cell response, but do not get processed from their protein and do not get presented.

Immunogenecity might not be the best sole criteria for the basis of vaccines, epitopes need to be protective too. This is hard but doable to figure out.

Organoids might be a way forward, but not the answer to everything (but could be the answer to a lot of things)

The spleen harbor a collection of different immune cells, and contain memory of infection and vaccination.

Next topic: two classes of regulatory T cells: CD4 Foxp3 T cells regulating B cells and a CD8 regulatory subset using granzyme B regulating T cell auto reactivity.

Exposure to pathogens (Mtb, CMV,...) remodels our immune system in a way that benefits them (and may hurt us)

Within TCR repertoires are motif clusters linked to disease progression, suggesting the existence of 'distractopes', epitopes that the disease wants you to react to, as a 'smoke screen' to redirect or exhaust the immune system.

Investigating the way that Mtb evades the immune system lead to the innovation of looking at the TCRs from tetramer sorting experiments.

Second topic: failures in vaccine trials. An infectious disease only becomes 'famous' when it learns how to evade our immune system.

First topic: how do human immune systems compare to inbred mice? Mice are the 'starter car', simple but lacking complexity, leading to failure in mice models.