TNFR2+ Tregs displayed stronger suppressive suppression and survival, together with higher resistance to oxidative stress (induced with menadione), and less intracellular ROS content, from spleen and tumors.

TNFR2+ and TNF+ Tregs partially maintained their phenotype when cultured in vitro. However, when cocultured, the TNFR2+ prevailed, and "transmitted" their phenotype (and their dye) to the TNF+ Tregs

We found TNF-producing Tregs in multiple organs in naive mice, as well as in tumors and in animals with arthritis. TNF production was a preferential feature of those Tregs not expressing the TNFR2.

Our study "Iron capture through CD71 drives perinatal and tumor-associated Treg expansion" is available at @jci-insight.bsky.social

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