8/8 🙏 Many thanks to all the contributors of this study !
@csavin.bsky.social @julmini.bsky.social
@yersiniaunit.bsky.social @pasteur.fr @chrudetours.bsky.social @univparissaclay.bsky.social @cea.fr @ap-hp.bsky.social @natureportfolio.nature.com @natcomms.nature.com

7/8 - Conclusion
💊 A fascinating case of in-host bacterial evolution — and a warning about long-term antibiotic exposure.

6/8 - Selection
🚫 We also identified positive selection signals:
- a truncation in the phosphate transporter PitA which probably impairs its activity
- an amino acid substitution interfering with the active site and diminishing the catalytic activity of the acyl-CoA thioesterase II TesB

5/8 - Metabolic adaptation
🍔 Omics analysis also unraveled:
- a progressive genome reduction
- metabolic changes in amino acid and carbon utilization
- a disturbed stringent response
which could contribute to the observed slower growth and antibiotic tolerance preceding resistance.

4/8 - Antibiotics resistance mechanisms
🧪 By analysing Ye.1 to Ye.4 genomes and proteomes, we could identify:
- a novel gyrA mutation leading to quinolone resistance
- ceftriaxone resistance via OmpF loss and β-lactamase activity

3/8 - Phylogenetic reconstruction
🧫 Ye.1 to Ye.4 belong to the Ye genotype 4 (bioserotype 4/O:3) and a form a unique clade separated from other Ye strains, strongly suggesting that the patient suffered from a chronic infection due to a unique evolving Ye 4/O:3 strain.

2/8 - Clinical case
🏥 Thanks to the National Reference Center for Plague and other Yersiniosis in @pasteur.fr, we collected 4 clinical isolates — Ye.1 to Ye.4, which evolved antibiotics tolerance and resistance — from a rare chronic infection in an elderly patient from @chrudetours.bsky.social.

Additional disclaimer: we did not SOLVE a mystery, it is just another small brick added on the top of all the knowledge accumulated since then...

A short animation to better understand our paper:
www.youtube.com/watch?v=o8nf...

More details here 👇https://bsky.app/profile/pierrelebury.bsky.social/post/3lqdcuo5gwk2v

12/12 🙏 Many thanks to the contributors of this study!
@ravneetksidhu.bsky.social @gmasfiol.bsky.social @bbolker.bsky.social @yersiniaunit.bsky.social
#ScienceResearch

11/12 - Impact
Our study highlights the importance of reservoir dynamics and structures to better understand virulence evolution of a zoonotic pathogen.