Next-generation orange-to-far-red photoconvertible fluorescent protein for single-molecule microscopy and protein dynamic tracking www.nature.com/articles/s41...

AAVs are crucial vectors for delivering biosensors into neurons! Using AAV delivery, we perform two-color imaging of calcium and potassium transients in neurons. These AAVs are now available from @braincase.bsky.social www.ebraincase.com/news/new-pro...

The hCRAFi-CCR2 sensor reports chemokine release in the mouse visual cortex after LPS challenge using two-photon microscopy www.nature.com/articles/s41.... Plasmids and corresponding test AAVs can be requested from WeKwikGene for free wekwikgene.wllsb.edu.cn/publications...

hCRAFi-CCR2 is a new chemokine receptor activation-based fluorescent indicator for monitoring spatiotemporal dynamics of CCR2 ligands, including human/mouse CCL2, and CCL7, as well as mouse CXCL1, in culture and in vivo www.nature.com/articles/s41...

PSmOrange3 works well for long-term mitochondria tracking in cultured mammalian cells. Plasmids for PSmOrange3 can be requested for free from @WeKwikGene here wekwikgene.wllsb.edu.cn/publications...

Temporal domain multiplexing enables multiplexed imaging in a single channel with spectrally similar FPs using per-pixel unmixing based on differences in photobleaching rates. To facilitate this approach, we developed new far-red FPs www.biorxiv.org/content/10.1...

Due to its high chemical stability and brightness, mBaoJin is a perfect FP for Expansion Microscopy. Three-dimensional view of expanded HeLa cell coexpressing H2B-mTurquoise2 and Mito (COX8Ax2)-mBaoJin (www.nature.com/articles/s41...)

Soma-targeting of GECIs significantly improves image segmentation, as can be seen from these examples of single-plane 2-photon imaging for FRCaMPi, jRGECO1a, and their soma-localized variant from L2/3 neurons of primary visual cortex in awake mouse journals.plos.org/plosbiology/...

mScarlet3-H (aka mYongHong) makes a great pair with mBaoJin (nature.com/articles/s41...) for long-term live cell imaging. All plasmids can be requested for free from WeKwikGene wekwikgene.wllsb.edu.cn