I am in total support of this method

Furthermore, we tracked the net CO₂ flux of germinating acorns (Quercus robur) until they completed their first flush. Finally, we conducted temperature response experiments with Q. robur seedlings to identify the temperature above which they have a negative net carbon balance.

We measured the net CO₂ flux of three C₃ and three CAM species over several days, as well as measuring the C₃ species over several days of continuous light and darkness, in order to investigate potential circadian rhythms.

To prove the reliability of the measurements and explore potential future experimental studies, we investigated net CO₂ fluxes in 4 experiments.

In most studies, the net C balance of the whole plant is modelled based on measurements of leaves, twigs and roots. However, measuring CO₂ fluxes of heterotrophic tissues is challenging, and CO₂ is redistributed inside the plant via sap flow.