Abhijith Babu
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phantomhelix.bsky.social
Abhijith Babu
@phantomhelix.bsky.social
Bioinformatician specializing in microbial genomics, with expertise in developing robust pipelines. Proficient in leveraging the Linux command line and shell scripting to optimize workflows and streamline complex processes.
⚠️ Your gut can make or break your health.
And the secret weapon? 👉 SCFAs (Short Chain Fatty Acids).
August 24, 2025 at 1:35 PM
Ever wondered what microbes are lurking in your environment? Here’s how scientists unravel their secrets with shotgun metagenomics!

1. Sample Collection & DNA Extraction

Collect environmental or clinical samples.

Extract total DNA, ensuring high quality and minimal contamination.
August 23, 2025 at 2:09 PM
16S amplicon sequencing analysis general workflow using DADA2:

We start with demultiplexed paired-end data (although single-end reads can also be analyzed).

If your data is not demultiplexed, you have to use an external program to demultiplex. Dada2 does not offer this functionality.
December 16, 2024 at 1:33 PM