Chase Morgan
@phagemorgan.bsky.social
MD/PhD student. Harm Reductionist. Here for the phages. All views my own.
Sure but the Juk in PA14 prevents KZ replication. Other juks probably protect against different phage. For example KZ and it's close relative PA3 share a replication mechanism but PA3 is insensitive to PA14 Juk.
December 14, 2024 at 6:34 PM
Sure but the Juk in PA14 prevents KZ replication. Other juks probably protect against different phage. For example KZ and it's close relative PA3 share a replication mechanism but PA3 is insensitive to PA14 Juk.
www.biorxiv.org/content/10.1... Discovery of Juk system in PA14 providing resistance to both PhiKZ and OMKO1 www.biorxiv.org/content/10.1... Knockdown of Juk restores PA14 PhiKZ sensitivity pubmed.ncbi.nlm.nih.gov/38963841/ and here we use PA14 to select out PhiKZ for quantification
A family of novel immune systems targets early infection of nucleus-forming jumbo phages
Jumbo bacteriophages of the ⌽KZ-like family are characterized by large genomes (>200 kb) and the remarkable ability to assemble a proteinaceous nucleus-like structure. The nucleus protects the phage g...
www.biorxiv.org
December 10, 2024 at 8:08 PM
www.biorxiv.org/content/10.1... Discovery of Juk system in PA14 providing resistance to both PhiKZ and OMKO1 www.biorxiv.org/content/10.1... Knockdown of Juk restores PA14 PhiKZ sensitivity pubmed.ncbi.nlm.nih.gov/38963841/ and here we use PA14 to select out PhiKZ for quantification
I'm not sure what MOI exactly means on a plate. 10^7 phage on a spot assay will make a clearing, but you don't get productive infection. I use PA14 to select against phiKZ during competition experiments because it's more or less completely resistant. I'll drop a few papers in a second.
December 10, 2024 at 8:03 PM
I'm not sure what MOI exactly means on a plate. 10^7 phage on a spot assay will make a clearing, but you don't get productive infection. I use PA14 to select against phiKZ during competition experiments because it's more or less completely resistant. I'll drop a few papers in a second.