😍

Amazing! Congrats!

6/ Read more in our manuscript from the
Greninger Lab
at UW Virology
: medrxiv.org/cgi/content/.... Many people contributed to this work, including
@margaretgracemills.bsky.social, Ailyn Perez-Osorio, and
@pavitrarc.bsky.social.

Grateful to my amazing colleagues and coauthors for contributing to this work. And our collaborators across the pond who generously shared early versions of the ARTIC primers. /end

tl;dr The assay shows robust performance across a range of viral loads allowing sensitive recovery for RSV genomes from clinical specimens. Its sensitivity makes it suitable for use in clinical trials of antivirals and vaccines allowing early detection of treatment emergent/resistance mutation. 4/

We determined assay sensitivity, specificity, repeatability and reproducibility; and comparison with Sanger, shotgun, and capture-based sequencing methods to check for accurate recovery of the F gene. We also used contrived samples to determine the LOD of minor variants in allelic mixtures. 3/

We modified 2 ARTIC primers based on initial tests with samples from USA (WA state), but changes are likely improve performance in global isolates based on current variation at those sites. We were able to recover high quality genomes from clinical samples with a wide range of Cts (95% LOD Ct 30) 2/

My brain as soon as it saw this pop up on my feed: "In the shuffling madness of the locomotive breath" 🚂

My dad introduced us to Jethro Tull, listening to these on audio cassettes in the car. Love every single song.

I think you probably meant this Evan Newell--I don't think he's on bsky.

www.fredhutch.org/en/faculty-l...