nyuhki
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nyuhki.bsky.social
nyuhki
@nyuhki.bsky.social
Biologist for Reproductive Medicine
Osaka | Kyoto | Berkeley
https://nyuhki.github.io/YukiNoguchi/
9/22(月)に出版される 「実験デザインからわかるCRISPRスクリーニング実践テキスト 」の
「精子機能を標的とする in vivo sgRNA ライブラリスクリーニングの方法論」を執筆させて頂きました。ご興味あればぜひご覧ください~

www.yodosha.co.jp/jikkenigaku/...
実験医学別冊:実験デザインからわかるCRISPRスクリーニング実践テキスト〜実験規模の設定から、表現型アッセイの組み立て、遺伝子同定まで、自在にカスタマイズする極意とプロトコール
遺伝子探索の画期的手法として注目されるCRISPRスクリーニングを解説した,本邦初の実験書.基本フローと考え方のポイントから実際のプロトコールまで丁寧に解説され,開発者ならではのノウハウも満載です.
www.yodosha.co.jp
September 21, 2025 at 7:48 AM
Reposted by nyuhki
If you would like to find out more about testicular in vivo revival screening, the method is detailed in this Star Protocol paper: doi.org/10.1016/j.xp... 🦠💉🐀
Redirecting
doi.org
March 14, 2025 at 6:16 AM
Reposted by nyuhki
Not only can revival screening be used in vitro, it can also be used in vivo! 🐀

Using in vivo revival screening, Noguchi-san identified Rd3 as a significant factor in spermatogenesis

doi.org/10.1016/j.xg...
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doi.org
March 14, 2025 at 6:13 AM
Reposted by nyuhki
Suzuki Lab has moved to Bluesky!
Want to find out more about our lab? We’ve summarized current screening systems, including revival screening, a key technique developed in our lab, here: doi.org/10.1146/annu... 🔑
Genome-Wide Screening Approaches for Biochemical Reactions Independent of Cell Growth | Annual Reviews
Genome-wide screening is a potent approach for comprehensively understanding the molecular mechanisms of biological phenomena. However, despite its widespread use in the past decades across various bi...
doi.org
March 14, 2025 at 6:01 AM