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notlost12.bsky.social
@notlost12.bsky.social
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The discovery is the result of collaboration between Swansea University, Freie Universität Berlin, and Charité-Universitätsmedizin Berlin.

The study has been published in Small. YES, it is PEER-REVIEWED.
• onlinelibrary.wiley.com/doi/10.1002/...

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GRAPHICAL ABSTRACT Both sulfated and sialylated glycans present on the cell surface act as co-receptors for the SARS-CoV-2 attachment. Polysialylated and sulfated dendritic polyglycerols are studied for their binding with SARS-CoV-2. In solution, dendritic polyglycerol sialosides (dPGSAs) bind significantly stronger (low nm) to SARS-CoV-2 than their sulfated analogs. dPGSAs effectively inhibit the viral entry into Calu-3 cells. ABSTRACT Both polysialosides and polysulfates are known to interact with the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. However, a comprehensive site by site analysis of their binding affinities and potential synergistic antiviral effects have not been performed. Here, we report on the synthesis of polysialosides with nanomolar binding affinities to spike proteins of SARS-CoV-2 in solution using microscale thermophoresis. The dendritic polyglycerol based polysialosides dPG500SA0.55 and dPG500SA0.25, with a dissociation constant Kd of 4.78 nm and 10.85 nm, respectively, bind ≥500 times stronger than the high density polysulfated analog dPG500S0.55, to intact SARS-CoV-2 virus particles or isolated spike protein. In fact, the presence of sulfate groups in a heteromultivalent compound dPG500SA0.20S0.20 weakens the binding to spike proteins. A polycarboxylated analog does not bind to SARS-CoV-2, ruling out that the interaction of polysialoside is simply driven by electrostatics. Using explicit-solvent all-atom molecular dynamics simulations and ensemble docking studies, atomistic details are obtained on the interaction of different functional groups with the SARS-CoV-2 RBD. The data support the conclusion that sialosides interact stronger than sulfates for their binding with RBD of SARS-CoV-2. Notably, the most affine binder dPG500SA0.55 inhibits SARS-CoV-2 (WT, D614G) replication up to 98.6% at 0.5 um concentrations.
August 12, 2025 at 4:02 PM
This says New Mexico...
April 23, 2025 at 2:00 PM
Thank for continuing on
March 19, 2025 at 12:53 AM
So disappointed. His post on facebook are far ranging.
Thank goodness he's at least on here
March 19, 2025 at 12:51 AM
So shocked you are off Facebook.
So relieved you are here
March 19, 2025 at 12:50 AM
Does this " pcr" testing include the German made personal tester called plus- life?
I know quite a few folks using it & they believe it catches it within 12to 24 hours of exposure (&infection)
February 2, 2025 at 2:06 PM