If you do not have access to the article please drop me a message, I am happy to share a link!

I was wondering first if it is autofluorescent, but grains that incubated longer had brighter signal which made me think that they were actually stained. But I would not exclude it completely.

As a member of the #Helfrich-Förster Lab, a staining against PDF cannot be missed :D
Enjoy another picture from the category #didn'tMakeItIntoThePaper-TooPrettyNotToBeShown #FluorescenceFriday 2/2

As a member of the Helfrich-Förster Lab, a staining against Pigment Dispersing Factor cannot be missed :D Enjoy another picture from the category #didn'tMakeItIntoThePaper-TooPrettyNotToBeShown #FluorescenceFriday 2/2

In this time-lapse video you can follow the first moments of an eclosing P. gymnostoma.

The cells near the accessory medulla resemble the PDF-positive LNv of D. melanogaster. This fly also shows PDH-ir cells in the last abdominal segment. Interestingly, P. gymnostoma has no fully condensed ganglia. The last abdominal ganglion is located much further in the abdomen than in Drosophila.

Starting off with this Drosophila brain expressing MultiColorFlipOut (Nern et al. 2015) in combination with Timeless-Gal4, which was created during the work for my last publication (doi.org/10.1038/s414...).
Picture was taken with the Sp8 @leicamicrosystems.bsky.social Confocal.