Nick McCurtin (they/them) 🧫 🏳️🌈
@nicholasmccurtin.bsky.social
Chemistry PhD candidate in the Scheck lab at Tufts University, pop culture enthusiast, amateur chef, dilettante aesthete...
Extreme rookie question, but our lab just had a power outage and now our #Orbitrap Exploris 240 is not acquiring any data. Here's what our HeLa digest standard looks like before and after outage. Anyone know what might be going on? #teammassspec #proteomics #chemsky #scisky
July 10, 2025 at 5:05 PM
Extreme rookie question, but our lab just had a power outage and now our #Orbitrap Exploris 240 is not acquiring any data. Here's what our HeLa digest standard looks like before and after outage. Anyone know what might be going on? #teammassspec #proteomics #chemsky #scisky
Campus-wide power outage? That's fine. We'll go out for ice cream instead!
July 1, 2025 at 1:18 AM
Campus-wide power outage? That's fine. We'll go out for ice cream instead!
Extremely niche troubleshooting request, but is anyone familiar with implementing MSFragger in Proteome Discoverer? A few of us keep seeing these error messages, but none of us are tech savvy enough to decode it. Every time we "solve" it, it happens again.. #proteomics #teammassspec
April 5, 2025 at 8:50 PM
Extremely niche troubleshooting request, but is anyone familiar with implementing MSFragger in Proteome Discoverer? A few of us keep seeing these error messages, but none of us are tech savvy enough to decode it. Every time we "solve" it, it happens again.. #proteomics #teammassspec
It's happening again! These are the same sample, just different biological replicates. What could make my peptides elute like that? #chemsky #proteomics #teammassspec
April 4, 2025 at 7:35 PM
It's happening again! These are the same sample, just different biological replicates. What could make my peptides elute like that? #chemsky #proteomics #teammassspec
Really unusual signal in some recent samples I injected on Orbitrap Exploris... Anyone ever seen something like this before? #teammassspec #chemsky #proteomics
March 31, 2025 at 2:56 PM
Really unusual signal in some recent samples I injected on Orbitrap Exploris... Anyone ever seen something like this before? #teammassspec #chemsky #proteomics
If I'm following, this could look like incubating post-digest beads with a harsh elution buffer, then looking at Coomassie for presence of protein (to diagnose if something's wrong with the digest step)? I'm seeing some laddering on TIC in the last 10 mins of my run, avg'd spectra looks like this.
January 11, 2025 at 10:21 PM
If I'm following, this could look like incubating post-digest beads with a harsh elution buffer, then looking at Coomassie for presence of protein (to diagnose if something's wrong with the digest step)? I'm seeing some laddering on TIC in the last 10 mins of my run, avg'd spectra looks like this.