Blake Russell
@neurorussell.bsky.social
PhD student in Lak Lab & lecturer @ the University of Oxford. Using multiphoton optogenetics and Neuropixels simultaneously to study how perceptual activity propagates through the brain.
Freelance 3D animation. DM for a quote
Freelance 3D animation. DM for a quote
Reposted by Blake Russell
Desensitization of opsin responses during all-optical interrogation depends on imaging parameters https://www.biorxiv.org/content/10.1101/2025.10.02.680058v1
October 4, 2025 at 12:16 AM
Desensitization of opsin responses during all-optical interrogation depends on imaging parameters https://www.biorxiv.org/content/10.1101/2025.10.02.680058v1
🚨New pre-print!🚨
Can we truly independently read and write neuronal activity at the same time using all-optical techniques? Here we show that even two-photon imaging over large fields of view with low dwell time per cell can bias neural dynamics!
Read on for some potential fixes: (1/5)
Can we truly independently read and write neuronal activity at the same time using all-optical techniques? Here we show that even two-photon imaging over large fields of view with low dwell time per cell can bias neural dynamics!
Read on for some potential fixes: (1/5)
Desensitization of opsin responses during all-optical interrogation depends on imaging parameters
Significance The combination of two-photon calcium imaging and two-photon optogenetic stimulation, termed all-optical interrogation, provides spatial and temporal precision when recording and manipula...
www.biorxiv.org
October 15, 2025 at 4:47 PM
🚨New pre-print!🚨
Can we truly independently read and write neuronal activity at the same time using all-optical techniques? Here we show that even two-photon imaging over large fields of view with low dwell time per cell can bias neural dynamics!
Read on for some potential fixes: (1/5)
Can we truly independently read and write neuronal activity at the same time using all-optical techniques? Here we show that even two-photon imaging over large fields of view with low dwell time per cell can bias neural dynamics!
Read on for some potential fixes: (1/5)
Reposted by Blake Russell
All-optical experiments are great but @neurorussell.bsky.social found that opsin desensitization due to cross-talk between the imaging laser and the opsin causes major issues. These can be ameleorated with long inter-stimulus intervals & short imaging doses. All here: www.biorxiv.org/content/10.1...
Desensitization of opsin responses during all-optical interrogation depends on imaging parameters
Significance The combination of two-photon calcium imaging and two-photon optogenetic stimulation, termed all-optical interrogation, provides spatial and temporal precision when recording and manipula...
www.biorxiv.org
October 12, 2025 at 12:27 PM
All-optical experiments are great but @neurorussell.bsky.social found that opsin desensitization due to cross-talk between the imaging laser and the opsin causes major issues. These can be ameleorated with long inter-stimulus intervals & short imaging doses. All here: www.biorxiv.org/content/10.1...