Neşe Çakmak
@neskacakmak.bsky.social
Research scientists with a focus on cancer immunotherapy and a passion for immunology research.
Based at the Max Delbrück Center for Molecular Medicine, Berlin
Based at the Max Delbrück Center for Molecular Medicine, Berlin
The Greenberg paper from 2014 in Nature protocols is a good one. Here is the link: www.nature.com/articles/npr...
Antigen-specific activation and cytokine-facilitated expansion of naive, human CD8+ T cells - Nature Protocols
Nature Protocols - Antigen-specific activation and cytokine-facilitated expansion of naive, human CD8+ T cells
www.nature.com
January 7, 2025 at 8:49 PM
The Greenberg paper from 2014 in Nature protocols is a good one. Here is the link: www.nature.com/articles/npr...
This paper is a detailed protocol for expanding and sorting antigen specific T cells after in vitro priming with autologous DCs: pubmed.ncbi.nlm.nih.gov/24675735/
Antigen-specific activation and cytokine-facilitated expansion of naive, human CD8+ T cells - PubMed
Antigen-specific priming of human, naive T cells has been difficult to assess. Owing to the low initial frequency in the naive cell pool of specific T cell precursors, such an analysis has been obscur...
pubmed.ncbi.nlm.nih.gov
November 24, 2024 at 1:38 PM
This paper is a detailed protocol for expanding and sorting antigen specific T cells after in vitro priming with autologous DCs: pubmed.ncbi.nlm.nih.gov/24675735/
It is doable. I didn't sort T cells with tetramers after ex vivo stimulation, but I know some colleagues were doing it. One could also do IFNg capture assay if TCR downregulation is a concern.
November 24, 2024 at 1:14 PM
It is doable. I didn't sort T cells with tetramers after ex vivo stimulation, but I know some colleagues were doing it. One could also do IFNg capture assay if TCR downregulation is a concern.