Natálie Palková
@nataliepalkova.bsky.social
New protocol, new image!
Optimized LR White embedding of U2OS cells for immunogold labeling.
Quite tricky embedding, since it is necessary to find a suitable way of LR White polymerization and avoid oxygen. In this case, the resin polymerized in a capsule placed on a coverslip for three days at 50°C
Optimized LR White embedding of U2OS cells for immunogold labeling.
Quite tricky embedding, since it is necessary to find a suitable way of LR White polymerization and avoid oxygen. In this case, the resin polymerized in a capsule placed on a coverslip for three days at 50°C
December 23, 2024 at 7:19 PM
New protocol, new image!
Optimized LR White embedding of U2OS cells for immunogold labeling.
Quite tricky embedding, since it is necessary to find a suitable way of LR White polymerization and avoid oxygen. In this case, the resin polymerized in a capsule placed on a coverslip for three days at 50°C
Optimized LR White embedding of U2OS cells for immunogold labeling.
Quite tricky embedding, since it is necessary to find a suitable way of LR White polymerization and avoid oxygen. In this case, the resin polymerized in a capsule placed on a coverslip for three days at 50°C
How to start my Bluesky? How else but with the nucleolus!
U2OS cell fixed with formaldehyde & glutaraldehyde, stained with OsO4, potassium ferricyanide and uranyl acetate, dehydrated with ethanol series and acetone, epon-embedded.
U2OS cell fixed with formaldehyde & glutaraldehyde, stained with OsO4, potassium ferricyanide and uranyl acetate, dehydrated with ethanol series and acetone, epon-embedded.
December 8, 2024 at 11:14 AM
How to start my Bluesky? How else but with the nucleolus!
U2OS cell fixed with formaldehyde & glutaraldehyde, stained with OsO4, potassium ferricyanide and uranyl acetate, dehydrated with ethanol series and acetone, epon-embedded.
U2OS cell fixed with formaldehyde & glutaraldehyde, stained with OsO4, potassium ferricyanide and uranyl acetate, dehydrated with ethanol series and acetone, epon-embedded.