Attending #AIChE2025 #AIChE in Boston next week? Connect with us! The group is presenting 5 talks on Wednesday & Thursday!

Plan to attend #aiche2025 in Boston next month? Friends, alumni, colleagues, please visit the Tufts ChBE reception on Sunday night at 7 pm. #aiche

Finally, we show that engineered spores can be used to degrade plastic PET films #plasticdegradation

We compare coat proteins for surface availability and protection from inactivating agents (e.g., heat, protease), and their effect on sporulation efficiency. We also assess how different coat proteins can be co-utilized to increase display levels.

Allelic tagging allows us to examine the activation timing and strength of promoters associated with spore coat structural proteins.

In addition, this work investigates scarless allelic tagging of spore coat proteins using genome editing by co-transformation, analyzing factors affecting genome editing by congression.

Allelic tagging allows us to examine the activation timing and strength of promoters associated with spore coat structural proteins.

In addition, this work investigates scarless allelic tagging of spore coat proteins using genome editing by co-transformation, analyzing factors affecting genome editing by congression.

The coding scheme holds well under a variety of growth conditions and with different proteins in maintaining high activity while retaining low cellular burden. There’s so much more in this paper that took 5+ years to complete – so please look at the final version! (7/8)

We also show that CHI recodes less adversely affect levels of highly expressed host proteins compared to CAI recodes. The competition is also less severe with modestly expressed native proteins, further indicating that the primary competition is for translational resources. (6/8)

V. surprisingly, we find that the excess supply supported by each codon is additive! So, we developed a metric, Codon Health Index (CHI), to get good expression while minimizing burden on the host. New re-codes with this scheme are predictably less competitive for host resources. (5/8)

By assessing each codon carefully & independently, we find those for which supply & demand is delicately balanced–R/CGT, L/CTG, & P/CCG–that rate high on the traditional codon optimization schemes (e.g., CAI). For other codons, we find that there is excess capacity unused by cells. (4/8)

Often, people recode heterologous genes to look like highly expressed native genes to get strong expression – this is counterproductive and increases resource competition. We set out to identify coding schemes that minimally affect host fitness. (3/8)