This supports a "Central Sensing" model. The Bone Marrow isn't just a factory; it's a command center. It senses systemic signals and biases progenitor output (cDC2A vs cDC2B) to anticipate peripheral immune needs. (8/9)

Does this ontogeny matter for function? Yes. We show that progenitors express distinct TLRs, cytokine, and chemokine receptors before leaving the BM. Functional potential is hardwired early, not just acquired in the tissue. (7/9)

Navigating these subsets can be tricky. We compiled a "cheat sheet" of the defining surface markers, lineage tracers, and transcription factors that distinguish cDC2A, cDC2B, DC3, and tDCs. A resource for the field! (6/9)

To resolve the "alphabet soup" of DC subsets, we utilized lineage tracing (Clec9a, Zbtb46, LysM, hCD2). The data supports that cDC2As and cDC2Bs have distinct ontogenetic histories separate from the DC3 and tDC lineages. (5/9)

What drives this identity? Our SCENIC analysis identifies unique gene regulatory networks for each. While IRF8 defines pDC/cDC1, we see distinct regulons operating in the other subsets: HIC1/RUNX3 for cDC2As and NR1H3 for cDC2Bs. (4/9)

Are these subsets truly distinct? We integrated scRNA-seq datasets from multiple key studies. The verdict? cDC2A, cDC2B, DC3s, and tDCs are robustly separable based on their gene expression profiles. (3/9)

Historically, cDC2 heterogeneity was thought to be purely niche-driven. But is it? Here, we map the landscape of dendritic cell ontogeny, distinguishing conventional lineages (cDC1, cDC2) from the distinct developmental trajectories of pDC, tDC, and DC3s. (2/9)

Welcoming new lab members :) #immunology @maroiliopoulou.bsky.social @vvcorreia.bsky.social

#feminismisforall #happywomensday

Thrilled to share that my postdoc, Maro, has been awarded an ERA - Marie Curie Postdoctoral Fellowship! 🎉 Watching the lab’s people succeed is even more rewarding than personal achievements. Huge congratulations, Maro—well deserved! 👏 #MarieCurieFellowship @maroiliopoulou.bsky.social

Celebrating successful grant postdoc application 🥂 @maroiliopoulou.bsky.social @champalimaudr.bsky.social

Thrilled about receiving the EHA Kick-Off Grant 2024 from the European Hematology Association. This grant will fund research in the nutrition-immunity axis that mediates conventional dendritic cell development in the bone marrow. 🙏 Thank you EHA for funding our research 🔬