Playing around with how to represent volume in #Snouty light sheet data. Here is a dividing hiPSC with endogenously tagged histone in all three orthogonal projections. Bluer is further away. If anything, it distracts from politics.

... and a projection perpendicular to the light sheet, so not truly a view from the top but at 38 (or 52) degrees ... this is still giving me a headache.

Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.

Finally having some time to play with ‘Snouty’ again. Thanks a million once more @tanner-fadero.bsky.social and @amsikking.bsky.social

Vaccine samples from the 1880s in Louis Pasteur’s laboratory in Arbois … Vaccines work!

Got stentor … thanks @wallaceucsf.bsky.social

At the North Bay Science Discovery Day today in Santa Rosa

Crowd is forming in SF #standupforscience

Highlighting our recent paper on how we think doublecortin (green) contributes to growth cone biomechanics in developing neurons for #FluorescenceFriday

www.cell.com/current-biol...

Been dragged to the fan expo with my son today … guess better than writing grants

Ever wondered which of the new green fluorescent proteins you should use for live cell microscopy? 🧪
Maybe there is an answer:
biorxiv.org/content/10.1...

No videos here ... yet. So, here is a cute AlphaFold2 model of the mEmerald labelled nanocages that we used to determine that there are ~120 DCX molecules bound per µm growth cone microtubule🧪
doi.org/10.1101/2024...

A huge shout out to Tanner Fadero who helped me immensely with Snouty alignment yesterday. A piece of lens paper fits barely in between!!