We show that these iPSC derived alveolar macrophages, which we call AM-L (like), resemble bonafide AMs at baseline and with Mtb infection, leveraging the insights of our collaborators in the Hawn lab published here:
journals.aai.org/jimmunol/art...

To faciliate human AM studies, we derived macrophage subtypes from iPSCs under distinct cytokines, including GM-CSF, which is required for development of AMs.

And also show that placing the ALFA tag at different places in a multidomain protein (Pks13) allows for domain specific interactomes:

And can be used for proximity proteomics by fusing NBALFA to TurboID. We present a full workflow for proximity proteomics in M. smegmatis and M. tuberculosis. Here shown for the mycolic acid biosynthetic protein Pks13:

Allison reasoned that placing the ALFA tag on the target protein and expressing the high affinity anti-ALFA nanobody fused to various functional domains in live mycobacteria would be a versatile system. We show that such an approach enables faitful localization of proteins, here shown for MmpL3...

Agree, very fun. I set the word frequency to 3. 🦠 #microsky

Important point, the name is misleading, as most names are. Here is the cdc map from 2019 for spotted fever group rickettsiosis.