Widefield imaging of cholinergic signaling revealed selective, visually evoked ACh release after visual fear conditioning. Local blockade of ACh muscarinic receptors in V1 disrupted both enhanced visual responses and conditioned behavior. ACh is required for neural and behavior plasticity! 3/5

In head-fixed mice, associating a visual cue with mild foot-shock resulted in conditioned cessation of licking (~freezing). Behavior required V1 activity, and wide field+2p calcium imaging showed learning-dependent increases in visual responses for V1 pyramidal neurons.

...and tonight, cookies!

Unbelievably thankful for being able to work alongside such a supportive, smart, enthusiastic group of scientists! Happy Holidays from the Higley Lab!

Cardin/Higley labs out for dinner at SFN! A few folks missing, but great to meet up outside work!