@mfeltes.bsky.social
Check it out in #PLOSGenetics: journals.plos.org/plosgenetics...
Phenotype to genotype: A new and rapid approach using whole-genome sequencing
Author summary Forward genetic mutagenesis screening is an unbiased approach for the identification of mutations linked to a phenotype of interest. While this approach can be a powerful tool for uncov...
journals.plos.org
July 15, 2025 at 5:42 PM
Check it out in #PLOSGenetics: journals.plos.org/plosgenetics...
We've used WheresWalker to map our newly discovered #darkyolk zebrafish mutants which have defects in #ApoB-lipoprotein metabolism. We map zion to slc3a2a, a heterodimeric amino acid transporter, and find that several SLC3a2/SLC7s are associated with ApoB phenotypes in humans.
July 15, 2025 at 5:42 PM
We've used WheresWalker to map our newly discovered #darkyolk zebrafish mutants which have defects in #ApoB-lipoprotein metabolism. We map zion to slc3a2a, a heterodimeric amino acid transporter, and find that several SLC3a2/SLC7s are associated with ApoB phenotypes in humans.
Validated in #zebrafish & #maize, this pipeline uses a combination of #mapping-by-sequencing and #recombinantmapping to narrow the number of candidate genes which can be tested using #F0crispr
July 15, 2025 at 5:42 PM
Validated in #zebrafish & #maize, this pipeline uses a combination of #mapping-by-sequencing and #recombinantmapping to narrow the number of candidate genes which can be tested using #F0crispr
Read more about the screen that led to our mutant discoveries, and the mapping tool (#WheresWalker) we're using to map our loci here: www.biorxiv.org/content/10.1...
WheresWalker: a pipeline for rapid mutation mapping using whole genome sequencing
Forward genetic screening is a powerful approach to assign functions to genes and can be used to elucidate the many genes whose functions remain unknown. Chemical mutagenesis is an unbiased and effici...
www.biorxiv.org
April 28, 2025 at 8:34 PM
Read more about the screen that led to our mutant discoveries, and the mapping tool (#WheresWalker) we're using to map our loci here: www.biorxiv.org/content/10.1...
Using forward genetic screening, we discovered 28 novel zebrafish mutants
@zebrafishrock.bsky.social
and named them after our parks
@altnps.bsky.social
🏞️🏞️. Our adventure into this biological wilderness is just beginning - we can't wait to share what we'll discover.
@zebrafishrock.bsky.social
and named them after our parks
@altnps.bsky.social
🏞️🏞️. Our adventure into this biological wilderness is just beginning - we can't wait to share what we'll discover.
April 28, 2025 at 8:32 PM
Using forward genetic screening, we discovered 28 novel zebrafish mutants
@zebrafishrock.bsky.social
and named them after our parks
@altnps.bsky.social
🏞️🏞️. Our adventure into this biological wilderness is just beginning - we can't wait to share what we'll discover.
@zebrafishrock.bsky.social
and named them after our parks
@altnps.bsky.social
🏞️🏞️. Our adventure into this biological wilderness is just beginning - we can't wait to share what we'll discover.