Margaret Schroeder
@meschro.bsky.social
neuroscience postdoc in the Darnell Lab at Rockefeller 👩🔬 | astrocytes 🧠⭐ | surviving synovial sarcoma🎗
Wow, so fun to think back to that! Thank you so much, Ed, for all your help along the way!
November 21, 2025 at 11:31 AM
Wow, so fun to think back to that! Thank you so much, Ed, for all your help along the way!
Code on GitHub (github.com/Feng-Lab-MIT...) and images on BossDB (bossdb.org/project/schr...).
GitHub - Feng-Lab-MIT/AstrocyteHeterogeneity: scripts for analysing snRNAseq, FISH, and ExR data accompanying Schroeder et al., 2025
scripts for analysing snRNAseq, FISH, and ExR data accompanying Schroeder et al., 2025 - Feng-Lab-MIT/AstrocyteHeterogeneity
github.com
November 20, 2025 at 6:33 PM
Code on GitHub (github.com/Feng-Lab-MIT...) and images on BossDB (bossdb.org/project/schr...).
Raw data (fastqs, h5 count matrices) on NeMO (data.nemoarchive.org/biccn/grant/...) and GEO (www.ncbi.nlm.nih.gov/geo/query/ac...).
Index of /biccn/grant/u01_feng/feng/transcriptome/sncell/10x_v3.1
data.nemoarchive.org
November 20, 2025 at 6:33 PM
Raw data (fastqs, h5 count matrices) on NeMO (data.nemoarchive.org/biccn/grant/...) and GEO (www.ncbi.nlm.nih.gov/geo/query/ac...).
Processed data on the Broad Institute Single Cell Portal, searchable and interactive: singlecell.broadinstitute.org/single_cell/... (mouse) and singlecell.broadinstitute.org/single_cell/... (marmoset).
A multi-region transcriptomic atlas of developmental cell type diversity in mouse brain - Single Cell Portal
We used 10x Genomics Chromium single-nucleus RNA sequencing to characterize the molecular diversity of brain cells across six developmental stages (from late fetal to aged adult) and four brain regions (prefrontal cortex, motor cortex, striatum, and thalamus) in the mouse brain. Each developmental time point and region combination (except for aged) has 3 biological replicate donors. Nuclei are NOT enriched (e.g., FACS sorted) for any particular population and contain neurons, glia, and vasculature populations.
singlecell.broadinstitute.org
November 20, 2025 at 6:33 PM
Processed data on the Broad Institute Single Cell Portal, searchable and interactive: singlecell.broadinstitute.org/single_cell/... (mouse) and singlecell.broadinstitute.org/single_cell/... (marmoset).
Though we focused our analysis on astrocytes, we did not sort or select for any particular cell type in our prep. Our dataset is a rich, publicly available resource containing all neural cell types - please explore via the links below, and feel free to reach out with any questions!
November 20, 2025 at 6:33 PM
Though we focused our analysis on astrocytes, we did not sort or select for any particular cell type in our prep. Our dataset is a rich, publicly available resource containing all neural cell types - please explore via the links below, and feel free to reach out with any questions!
We then virally labeled astrocytes with GFP and used expansion microscopy to visualize their detailed morphology (it was so fun to take these images!). We quantified their morphological differences across regions in mouse.
November 20, 2025 at 6:33 PM
We then virally labeled astrocytes with GFP and used expansion microscopy to visualize their detailed morphology (it was so fun to take these images!). We quantified their morphological differences across regions in mouse.
Looking across species, we found broad conservation of cell types. However, several marmoset cell types appeared more mature than their mouse counterparts at birth, and the gene expression signatures underlying astrocyte regional heterogeneity diverged significantly between mouse and marmoset.
November 20, 2025 at 6:33 PM
Looking across species, we found broad conservation of cell types. However, several marmoset cell types appeared more mature than their mouse counterparts at birth, and the gene expression signatures underlying astrocyte regional heterogeneity diverged significantly between mouse and marmoset.
As others had shown, we found that astrocytic regional heterogeneity is patterned embryonically. What we were surprised to find was just how much regional astrocyte gene expression signatures change over postnatal development, likely in support of local neuronal circuits.
November 20, 2025 at 6:33 PM
As others had shown, we found that astrocytic regional heterogeneity is patterned embryonically. What we were surprised to find was just how much regional astrocyte gene expression signatures change over postnatal development, likely in support of local neuronal circuits.
To answer this question, we profiled millions of single nuclei with 10x Genomics snRNAseq across brain regions (motor and prefrontal cortices, striatum, and thalamus) and developmental time (from late embryonic to aged time points) in mouse and marmoset.
November 20, 2025 at 6:33 PM
To answer this question, we profiled millions of single nuclei with 10x Genomics snRNAseq across brain regions (motor and prefrontal cortices, striatum, and thalamus) and developmental time (from late embryonic to aged time points) in mouse and marmoset.
What started out as an exploration of astrocyte regional heterogeneity in the young adult marmoset led us to ask, how early in development is astrocyte regional heterogeneity patterned, and how does it change over developmental time and across mammalian species?
November 20, 2025 at 6:33 PM
What started out as an exploration of astrocyte regional heterogeneity in the young adult marmoset led us to ask, how early in development is astrocyte regional heterogeneity patterned, and how does it change over developmental time and across mammalian species?