Monica McCallum
@memccallum.bsky.social
couch/tabletop gamer and least useful member of the trivia team | organic, biological, and microbial chemist | she/her | views/posts my own
Congratulations!!!
July 2, 2025 at 1:42 AM
Congratulations!!!
I done that with a multiple sequence alignment of representative target sequences and Geneious, but I am sure there are many ways to go about it! help.geneious.com/hc/en-us/art...
How do I design degenerate primers based on an alignment?
Using the Design New Primers operation
Geneious Prime can design degenerate primers based on an alignment using the Design New primers tool under the Primers menu. For example, if you want a degen...
help.geneious.com
March 27, 2025 at 1:53 AM
I done that with a multiple sequence alignment of representative target sequences and Geneious, but I am sure there are many ways to go about it! help.geneious.com/hc/en-us/art...
It is possible to design and order so-called "degenerate primers" for amplifying highly similar genes from a variety of sources. That might be the sort of thing you're looking for?
March 27, 2025 at 1:47 AM
It is possible to design and order so-called "degenerate primers" for amplifying highly similar genes from a variety of sources. That might be the sort of thing you're looking for?
Thanks Bill! I am curious to see if we'll someday find cryptic sulfations standing in for cryptic halogenations in some biosyntheses...
February 6, 2025 at 1:41 AM
Thanks Bill! I am curious to see if we'll someday find cryptic sulfations standing in for cryptic halogenations in some biosyntheses...