Each kit comes with enough reagents for I think 5 or 6 library preps so using 4 barcodes instead of 2 shouldn’t strain your resources too much. I have used the same V24 kit for many sequencing runs.

The reason for this I believe in part is pore occupancy. If it’s sample that you have a lot of DNA you could take each sample split it in two and use four barcodes. After the run you could then just pool the reads for the two barcodes. ONT calls for high input DNA but I’ve run it much lower.

You/your team should fact check your podcasts before they’re released. There are so many people go to you as a source of scientific truth.