@mathiaseder.bsky.social
Thank you @andersshansen.bsky.social and Jamie Drayton for this very nice collaboration and the beautiful RCMC maps 😀
September 20, 2025 at 11:35 AM
Thank you @andersshansen.bsky.social and Jamie Drayton for this very nice collaboration and the beautiful RCMC maps 😀
For another cool example of what “hopping” can uncover—this time in the context of lamina interactions—check out Lise’s paper: www.nature.com/articles/s41...
Interactions between the genome and the nuclear lamina are multivalent and cooperative - Nature Structural & Molecular Biology
Using a transposon-based approach to create a set of large genomic rearrangements, Dauban et al. demonstrate that interactions of lamina-associated domains with the nuclear lamina involve multiple con...
www.nature.com
September 19, 2025 at 2:10 PM
For another cool example of what “hopping” can uncover—this time in the context of lamina interactions—check out Lise’s paper: www.nature.com/articles/s41...
6/ Beyond Sox2, this technology opens the door to studying how genomic location shapes the activity of many different DNA elements in a high-throughput way. We believe it will be broadly useful for diverse biological questions. 🚀
September 19, 2025 at 2:09 PM
6/ Beyond Sox2, this technology opens the door to studying how genomic location shapes the activity of many different DNA elements in a high-throughput way. We believe it will be broadly useful for diverse biological questions. 🚀
5/ We found that this competition is partly encoded in the Sox2 coding sequence (CDS) – a 1kb exon. Addition of the CDS to the reporter increases reporter expression and competition with the Sox2 gene. This effect seems to be mediated by the CDS-DNA sequence rather than the produced RNA or protein.
September 19, 2025 at 2:09 PM
5/ We found that this competition is partly encoded in the Sox2 coding sequence (CDS) – a 1kb exon. Addition of the CDS to the reporter increases reporter expression and competition with the Sox2 gene. This effect seems to be mediated by the CDS-DNA sequence rather than the produced RNA or protein.
4/ Strikingly, deleting the endogenous Sox2 gene expands this landscape—yet the original Sox2 site remains a “sweet spot” for activation. Genes can thus act as gatekeepers, restricting their enhancer’s influence.
September 19, 2025 at 2:09 PM
4/ Strikingly, deleting the endogenous Sox2 gene expands this landscape—yet the original Sox2 site remains a “sweet spot” for activation. Genes can thus act as gatekeepers, restricting their enhancer’s influence.
3/ These maps depict a sharply confined activation landscape. Interestingly, reporter expression is highest at the enhancer and around the endogenous gene whereas it turns silent upstream of the gene and downstream of the enhancer.
September 19, 2025 at 2:09 PM
3/ These maps depict a sharply confined activation landscape. Interestingly, reporter expression is highest at the enhancer and around the endogenous gene whereas it turns silent upstream of the gene and downstream of the enhancer.
2/ By “loading” our transposon with a fluorescent Sox2 reporter, relocating it, and sorting cells by reporter expression, we generated high-resolution activity maps.
September 19, 2025 at 2:09 PM
2/ By “loading” our transposon with a fluorescent Sox2 reporter, relocating it, and sorting cells by reporter expression, we generated high-resolution activity maps.
1/ Using a transposon-based approach, we systematically studied the positional relationship between the mouse Sox2 gene and its enhancer (SCR). In a single experiment, we can map >2000 unique genomic positions.
September 19, 2025 at 2:09 PM
1/ Using a transposon-based approach, we systematically studied the positional relationship between the mouse Sox2 gene and its enhancer (SCR). In a single experiment, we can map >2000 unique genomic positions.