Great, I’ll start digging through our plasmid collection.

Hello Phil,

can you paste me the sequence please ? thank you

very interesting topic. Could someone share the transposon sequence so we can check whether it’s present in our plasmids?

I would be interested in getting the book chapter ;)
Thank you

me too ;)

Interesting, I have a lot of them in bad shape but do not how to resharpened them. Any protocols to share.
Thank you

Part 2. I have used Genscript for many years for syntheses ranging from 1kb to more than 15kb.
They have always gone out of their way to get it synthesised, even after several attempts. I have tried Twist who are competitive on price but not on their ability to synthesise complex fragments.

Part 1. The price per base also depends on the complexity of the fragment to be synthesised (e.g. repeats) that will increase the final bill.

Would be happy to join