Marson Lab
@marsonlab.bsky.social
News from Dr. Alex Marson's lab UCSF & Gladstone Institutes, focusing on advances in immunology, CRISPR & genomics.
We also show combinatorial genetic and epigenetic engineering with CRISPR Cas12a-dCas9 to perform targeted CAR knock-in with CRISPRoff-silencing of target genes to improve preclinical CAR T performance. Read the full paper here: rdcu.be/eL0GK
Integrated epigenetic and genetic programming of primary human T cells
Nature Biotechnology - Multiplexed editing in primary human T cells generates enhanced immune cell therapies.
rdcu.be
October 22, 2025 at 4:07 PM
We also show combinatorial genetic and epigenetic engineering with CRISPR Cas12a-dCas9 to perform targeted CAR knock-in with CRISPRoff-silencing of target genes to improve preclinical CAR T performance. Read the full paper here: rdcu.be/eL0GK
Using an all-RNA platform, we achieve efficient, durable, and multiplexed epigenetic programming in primary human T cells without the need for sustained expression of CRISPR machinery. The epigenetic changes are stable through cell divisions, cell stimulations, and after in vivo adoptive transfer.
Integrated epigenetic and genetic programming of primary human T cells
Nature Biotechnology - Multiplexed editing in primary human T cells generates enhanced immune cell therapies.
rdcu.be
October 22, 2025 at 4:07 PM
Using an all-RNA platform, we achieve efficient, durable, and multiplexed epigenetic programming in primary human T cells without the need for sustained expression of CRISPR machinery. The epigenetic changes are stable through cell divisions, cell stimulations, and after in vivo adoptive transfer.