Thank you Mart! 🙏

Huge thanks to the whole team, bringing together the NMR and crystallography platforms at @pasteur.fr, the @sauguet-lab.bsky.social and our collaborators at @ifremer.bsky.social 🙏

Our results led us to propose a mechanism by which RPA can stimulate primase activity in the lagging strand through its winged-helix domain. If you are interested in RPA-mediated molecular interactions in the replisome, check the paper linked above! 🧵(4/4)

We show that in our primer extension assays, RPA stimulates the DNA polymerase activity of the primase through its winged-helix domain. 🧵(3/4)

We present the winged-helix domain of Rpa2, which is responsible for archaeal RPA-mediated protein-protein interactions, in complex with the primase and the DNA polymerase PolD. Integrative structural biology approach combining NMR, X-ray and cryo-EM! 🧵(2/4)

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