Yes! More generally for affective and/or very salient outcomes, everything could be different. We only measured visual cortex in benign "daily life" scenarios.

Another way to think of it is novel items/situations versus novel temporal order of familiar items. However, it could be different in non-sensory brain areas, or in other species, or in other modalities.

TetO‐jGCaMP8s is a drop in replacement for the @crisniell.bsky.social mice with all the nice properties of 8s. High, dense expression, and doesn't have the aberrant activity. Was used here: www.nature.com/articles/s41...

Ok, yes, this one will be tough no matter what. It might work a little better if there was more context, for example if the FOV was larger.

Could you share an image please?

I don't think we have good examples to look at this, but you can test it directly on the website or in the GUI and let us know.

Cellpose-SAM is currently available in the GUI.

The Cellpose-SAM backbone, yes. The SAM encoder weights were re-trained on a lot of cellular data to predict the Cellpose flows.