Hannes Maib
@maiblab.bsky.social
Recent PI and Wellcome Trust fellow at the University of Sheffield. Combining microscopy with membrane reconstitution and cell biology. Lover of Phosphoinositides and rock climbing
Importantly it also allows us to directly compare beads with different surface functionalisation. Here I attached the constitutively active mutant of the small GTPase Arf6 (Arf6-QL) and the inactive mutant to beads and we see complete separate reaction kinetics!
November 18, 2024 at 4:37 PM
Importantly it also allows us to directly compare beads with different surface functionalisation. Here I attached the constitutively active mutant of the small GTPase Arf6 (Arf6-QL) and the inactive mutant to beads and we see complete separate reaction kinetics!
These realtime kinetics allow us to monitor individual reactions and revealed strong stochastic effects due to complex feedback loops as has been shown by Scott Hansen and Jay Groves www.pnas.org/doi/full/10....
November 18, 2024 at 4:37 PM
These realtime kinetics allow us to monitor individual reactions and revealed strong stochastic effects due to complex feedback loops as has been shown by Scott Hansen and Jay Groves www.pnas.org/doi/full/10....
In my first real post I want to show the key experiment that revealed to me what I want my own lab to be about:
The conversion of phosphoinositides!
By using fluorescent biosensors and membrane coated beads I was able to reconstitute the real time kinetics of PI(4)P to PI(4,5)P2 conversion by PIP5K
The conversion of phosphoinositides!
By using fluorescent biosensors and membrane coated beads I was able to reconstitute the real time kinetics of PI(4)P to PI(4,5)P2 conversion by PIP5K
November 18, 2024 at 4:37 PM
In my first real post I want to show the key experiment that revealed to me what I want my own lab to be about:
The conversion of phosphoinositides!
By using fluorescent biosensors and membrane coated beads I was able to reconstitute the real time kinetics of PI(4)P to PI(4,5)P2 conversion by PIP5K
The conversion of phosphoinositides!
By using fluorescent biosensors and membrane coated beads I was able to reconstitute the real time kinetics of PI(4)P to PI(4,5)P2 conversion by PIP5K