Thank you to John Baenziger, @hugbum.bsky.social, Corrie daCosta, and Lefteris Zarkadas who worked together to create this amazing story - I am so grateful for you all!
Thanks to Christian Tessier who performed the single-channel recordings and Anna Ananchenko and Francois Dehez for the MD

Structural and functional data. Overall, we propose that the nAChR activates through asynchronous conformation transitions that challenge previous paradigms of MWC type mechanisms in Cys-loop receptors.

While the remaining subunits remain inactive, albeit poised for activation. We confirm this state is non-conductive with MD simulations and use single channel recordings across a broad range of ACh concentrations to position it within an improved kinetic scheme that accurately describes both…