Luke Lambourne
@lukelambourne.bsky.social
Scientist at Dana-Farber Cancer Institute / Harvard Medical School
Reposted by Luke Lambourne
reposting the 🧵 from the other site that I wrote when the preprint came out:
TFs, like most genes, are frequently expressed as a series of multiple distinct isoforms. these isoforms (by definition) differ in sequence -- often in annotated protein domain regions (e.g. DNA-binding & effector domains)
TFs, like most genes, are frequently expressed as a series of multiple distinct isoforms. these isoforms (by definition) differ in sequence -- often in annotated protein domain regions (e.g. DNA-binding & effector domains)
March 26, 2025 at 6:05 PM
reposting the 🧵 from the other site that I wrote when the preprint came out:
TFs, like most genes, are frequently expressed as a series of multiple distinct isoforms. these isoforms (by definition) differ in sequence -- often in annotated protein domain regions (e.g. DNA-binding & effector domains)
TFs, like most genes, are frequently expressed as a series of multiple distinct isoforms. these isoforms (by definition) differ in sequence -- often in annotated protein domain regions (e.g. DNA-binding & effector domains)
The project was a huge multi-lab collaboration over several years. Thanks to @kaiamattioli.bsky.social @fuxmanlab.bsky.social @nathansalomonis.bsky.social @annaber.bsky.social @alexholehouse.bsky.social @mcalderwood.bsky.social and all the other authors not on bluesky!
March 26, 2025 at 6:38 PM
The project was a huge multi-lab collaboration over several years. Thanks to @kaiamattioli.bsky.social @fuxmanlab.bsky.social @nathansalomonis.bsky.social @annaber.bsky.social @alexholehouse.bsky.social @mcalderwood.bsky.social and all the other authors not on bluesky!
We've added a few things since the preprint: biophysical modeling of how differences between disordered regions can affect DNA binding, a deeper look at how the differences in molecular function between isoforms relate to differential isoform expression in cancer and development.
March 26, 2025 at 6:38 PM
We've added a few things since the preprint: biophysical modeling of how differences between disordered regions can affect DNA binding, a deeper look at how the differences in molecular function between isoforms relate to differential isoform expression in cancer and development.
We found that:
- 2/3 of alternative isoforms showed some difference from the reference
- These differences were often unpredictable from the aa sequence differences alone
- alt isoforms fell into two categories: Negative Regulators, and Rewirers
Data is browsable at: TFisoDB.org
- 2/3 of alternative isoforms showed some difference from the reference
- These differences were often unpredictable from the aa sequence differences alone
- alt isoforms fell into two categories: Negative Regulators, and Rewirers
Data is browsable at: TFisoDB.org
TFisoDB
TFisoDB.org
March 26, 2025 at 6:38 PM
We found that:
- 2/3 of alternative isoforms showed some difference from the reference
- These differences were often unpredictable from the aa sequence differences alone
- alt isoforms fell into two categories: Negative Regulators, and Rewirers
Data is browsable at: TFisoDB.org
- 2/3 of alternative isoforms showed some difference from the reference
- These differences were often unpredictable from the aa sequence differences alone
- alt isoforms fell into two categories: Negative Regulators, and Rewirers
Data is browsable at: TFisoDB.org
Reposted by Luke Lambourne
On Wednesday 2/26, I’ll be in Boston to give a Harvard Science Book Talk. I’ll have a marvelous relic of aerobiological history to unveil. Details here: www.harvard.com/event/carl-z... 3/5
www.harvard.com
February 19, 2025 at 5:46 PM
On Wednesday 2/26, I’ll be in Boston to give a Harvard Science Book Talk. I’ll have a marvelous relic of aerobiological history to unveil. Details here: www.harvard.com/event/carl-z... 3/5