Luis Hernández-Huertas
@luisherhuertas.bsky.social
Postdoctoral researcher at @crisprscan.bsky.social @cabd-upo-csic.bsky.social | Understanding developmental biology using CRISPR tools 🐟🧬✂️
Thanks to all the co-authors for their extensive work, and to all the funding agencies for supporting this project! (13/13)
November 20, 2025 at 3:14 PM
Thanks to all the co-authors for their extensive work, and to all the funding agencies for supporting this project! (13/13)
In sum:
✂️ CRISPR-RfxCas13d enables comprehensive maternal mRNA knockdown screens, uncovering new post-translational regulators of MZT in teleost🐟 (12/13)
✂️ CRISPR-RfxCas13d enables comprehensive maternal mRNA knockdown screens, uncovering new post-translational regulators of MZT in teleost🐟 (12/13)
November 20, 2025 at 3:14 PM
In sum:
✂️ CRISPR-RfxCas13d enables comprehensive maternal mRNA knockdown screens, uncovering new post-translational regulators of MZT in teleost🐟 (12/13)
✂️ CRISPR-RfxCas13d enables comprehensive maternal mRNA knockdown screens, uncovering new post-translational regulators of MZT in teleost🐟 (12/13)
💡 We additionally demonstrated a Bckdk–Phf10 interaction via co-immunoprecipitation in zebrafish embryos (11/13)
November 20, 2025 at 3:14 PM
💡 We additionally demonstrated a Bckdk–Phf10 interaction via co-immunoprecipitation in zebrafish embryos (11/13)
💡 Restoring Phf10 phosphorylation rescues both developmental defects and H3K27ac depletion (10/13)
November 20, 2025 at 3:14 PM
💡 Restoring Phf10 phosphorylation rescues both developmental defects and H3K27ac depletion (10/13)
Phospho-proteomics revealed Phf10/Baf45a, a protein of the pBAF complex of the SWI/SNF family, as a Bckdk target, with reduced threonine phosphorylation upon KD (9/13)
November 20, 2025 at 3:14 PM
Phospho-proteomics revealed Phf10/Baf45a, a protein of the pBAF complex of the SWI/SNF family, as a Bckdk target, with reduced threonine phosphorylation upon KD (9/13)
Applying Slam-Seq and RNA-seq we revealed that Bckdk depletion affects both zygotic genome activation, with a reduced H3K27ac (💡by CUT&RUN analysis), and maternal RNA clearance particularly through miR-430 that is less processed and active (8/13)
November 20, 2025 at 3:14 PM
Applying Slam-Seq and RNA-seq we revealed that Bckdk depletion affects both zygotic genome activation, with a reduced H3K27ac (💡by CUT&RUN analysis), and maternal RNA clearance particularly through miR-430 that is less processed and active (8/13)
💡 Crucially, kinase-dead Bckdk versions fail to rescue developmental defects after KD 👉 showing that Bckdk kinase activity is essential for early development (7/13)
November 20, 2025 at 3:14 PM
💡 Crucially, kinase-dead Bckdk versions fail to rescue developmental defects after KD 👉 showing that Bckdk kinase activity is essential for early development (7/13)
We identified Bckdk, a mitochondrial/cytosolic kinase, as a regulator of zygotic genome activation (ZGA) in zebrafish and medaka.
Notably, its mitochondrial role remains intact upon KD 👉 pointing to a non-mitochondrial function during MZT (6/13)
Notably, its mitochondrial role remains intact upon KD 👉 pointing to a non-mitochondrial function during MZT (6/13)
November 20, 2025 at 3:14 PM
We identified Bckdk, a mitochondrial/cytosolic kinase, as a regulator of zygotic genome activation (ZGA) in zebrafish and medaka.
Notably, its mitochondrial role remains intact upon KD 👉 pointing to a non-mitochondrial function during MZT (6/13)
Notably, its mitochondrial role remains intact upon KD 👉 pointing to a non-mitochondrial function during MZT (6/13)
Our RNP-based Cas13d system achieves highly specific & efficient mRNA depletion (median ~92% knockdown) outperforming our previous versions (5/13)
November 20, 2025 at 3:14 PM
Our RNP-based Cas13d system achieves highly specific & efficient mRNA depletion (median ~92% knockdown) outperforming our previous versions (5/13)
(💡= new in the paper) We included positive & negative controls (gfp/rfp and nanog KD) confirming the specificity and robustness of Cas13d-mediated mRNA knockdown in both zebrafish and medaka.
👉 Negative controls had no developmental or molecular effects (4/13)
👉 Negative controls had no developmental or molecular effects (4/13)
November 20, 2025 at 3:14 PM
(💡= new in the paper) We included positive & negative controls (gfp/rfp and nanog KD) confirming the specificity and robustness of Cas13d-mediated mRNA knockdown in both zebrafish and medaka.
👉 Negative controls had no developmental or molecular effects (4/13)
👉 Negative controls had no developmental or molecular effects (4/13)
Post-translational regulation during the MZT remains largely unexplored.
Here, we applied our in vivo optimized CRISPR-RfxCas13d tool (PMID: 32768421, PMID: 40091120) to perform a maternal screening of 49 kinases and phosphatases in zebrafish embryos (3/13)
Here, we applied our in vivo optimized CRISPR-RfxCas13d tool (PMID: 32768421, PMID: 40091120) to perform a maternal screening of 49 kinases and phosphatases in zebrafish embryos (3/13)
November 20, 2025 at 3:14 PM
Post-translational regulation during the MZT remains largely unexplored.
Here, we applied our in vivo optimized CRISPR-RfxCas13d tool (PMID: 32768421, PMID: 40091120) to perform a maternal screening of 49 kinases and phosphatases in zebrafish embryos (3/13)
Here, we applied our in vivo optimized CRISPR-RfxCas13d tool (PMID: 32768421, PMID: 40091120) to perform a maternal screening of 49 kinases and phosphatases in zebrafish embryos (3/13)
Using our optimized CRISPR-RfxCas13d screening strategy, we identified Bckdk as a previously unrecognized kinase controlling the maternal-to-zygotic transition (MZT) in teleosts 🐟 (2/13)
November 20, 2025 at 3:14 PM
Using our optimized CRISPR-RfxCas13d screening strategy, we identified Bckdk as a previously unrecognized kinase controlling the maternal-to-zygotic transition (MZT) in teleosts 🐟 (2/13)
And thanks to all the co-authors for their extensive work, and to all the funding agencies for supporting this project! ✨✨ (13/13)
November 20, 2025 at 3:04 PM
And thanks to all the co-authors for their extensive work, and to all the funding agencies for supporting this project! ✨✨ (13/13)
In sum:
✂️ CRISPR-RfxCas13d enables comprehensive maternal mRNA knockdown screens, uncovering new post-translational regulators of MZT in teleost🐟. (12/13)
✂️ CRISPR-RfxCas13d enables comprehensive maternal mRNA knockdown screens, uncovering new post-translational regulators of MZT in teleost🐟. (12/13)
November 20, 2025 at 3:04 PM
In sum:
✂️ CRISPR-RfxCas13d enables comprehensive maternal mRNA knockdown screens, uncovering new post-translational regulators of MZT in teleost🐟. (12/13)
✂️ CRISPR-RfxCas13d enables comprehensive maternal mRNA knockdown screens, uncovering new post-translational regulators of MZT in teleost🐟. (12/13)
💡 We additionally demonstrated a Bckdk–Phf10 interaction via co-immunoprecipitation in zebrafish embryos (11/13)
November 20, 2025 at 3:04 PM
💡 We additionally demonstrated a Bckdk–Phf10 interaction via co-immunoprecipitation in zebrafish embryos (11/13)
💡 Restoring Phf10 phosphorylation rescues both developmental defects and H3K27ac depletion (10/13)
November 20, 2025 at 3:04 PM
💡 Restoring Phf10 phosphorylation rescues both developmental defects and H3K27ac depletion (10/13)
Phospho-proteomics revealed Phf10/Baf45a, a protein of the pBAF complex of the SWI/SNF family, as a Bckdk target, with reduced threonine phosphorylation upon KD (9/13)
November 20, 2025 at 3:04 PM
Phospho-proteomics revealed Phf10/Baf45a, a protein of the pBAF complex of the SWI/SNF family, as a Bckdk target, with reduced threonine phosphorylation upon KD (9/13)
Applying Slam-Seq and RNA-seq we revealed that Bckdk depletion affects both zygotic genome activation, with a reduced H3K27ac (💡by CUT&RUN analysis), and maternal RNA clearance particularly through miR-430 that is less processed and active (8/13)
November 20, 2025 at 3:04 PM
Applying Slam-Seq and RNA-seq we revealed that Bckdk depletion affects both zygotic genome activation, with a reduced H3K27ac (💡by CUT&RUN analysis), and maternal RNA clearance particularly through miR-430 that is less processed and active (8/13)
💡 Crucially, kinase-dead Bckdk versions fail to rescue developmental defects after KD 👉 showing that Bckdk kinase activity is essential for early development (7/13)
November 20, 2025 at 3:04 PM
💡 Crucially, kinase-dead Bckdk versions fail to rescue developmental defects after KD 👉 showing that Bckdk kinase activity is essential for early development (7/13)
We identified Bckdk, a mitochondrial/cytosolic kinase, as a regulator of zygotic genome activation (ZGA) in zebrafish and medaka.
Notably, its mitochondrial role remains intact upon KD 👉 pointing to a non-mitochondrial function during MZT (6/13)
Notably, its mitochondrial role remains intact upon KD 👉 pointing to a non-mitochondrial function during MZT (6/13)
November 20, 2025 at 3:04 PM
We identified Bckdk, a mitochondrial/cytosolic kinase, as a regulator of zygotic genome activation (ZGA) in zebrafish and medaka.
Notably, its mitochondrial role remains intact upon KD 👉 pointing to a non-mitochondrial function during MZT (6/13)
Notably, its mitochondrial role remains intact upon KD 👉 pointing to a non-mitochondrial function during MZT (6/13)
Our RNP-based Cas13d system achieves highly specific & efficient mRNA depletion (median ~92% knockdown) outperforming our previous versions (5/13)
November 20, 2025 at 3:04 PM
Our RNP-based Cas13d system achieves highly specific & efficient mRNA depletion (median ~92% knockdown) outperforming our previous versions (5/13)
(💡 = new in the paper) We included positive & negative controls (gfp/rfp and nanog KD) confirming the specificity and robustness of Cas13d-mediated mRNA knockdown in both zebrafish and medaka.
👉 Negative controls had no developmental or molecular effects (4/13)
👉 Negative controls had no developmental or molecular effects (4/13)
November 20, 2025 at 3:04 PM
(💡 = new in the paper) We included positive & negative controls (gfp/rfp and nanog KD) confirming the specificity and robustness of Cas13d-mediated mRNA knockdown in both zebrafish and medaka.
👉 Negative controls had no developmental or molecular effects (4/13)
👉 Negative controls had no developmental or molecular effects (4/13)
Post-translational regulation during the MZT remains largely unexplored.
Here, we applied our in vivo optimized CRISPR-RfxCas13d tool (PMID: 32768421, PMID: 40091120) to perform a maternal screening of 49 kinases and phosphatases in zebrafish embryos (3/13)
Here, we applied our in vivo optimized CRISPR-RfxCas13d tool (PMID: 32768421, PMID: 40091120) to perform a maternal screening of 49 kinases and phosphatases in zebrafish embryos (3/13)
November 20, 2025 at 3:04 PM
Post-translational regulation during the MZT remains largely unexplored.
Here, we applied our in vivo optimized CRISPR-RfxCas13d tool (PMID: 32768421, PMID: 40091120) to perform a maternal screening of 49 kinases and phosphatases in zebrafish embryos (3/13)
Here, we applied our in vivo optimized CRISPR-RfxCas13d tool (PMID: 32768421, PMID: 40091120) to perform a maternal screening of 49 kinases and phosphatases in zebrafish embryos (3/13)
Using our optimized CRISPR-RfxCas13d screening strategy, we identified Bckdk as a previously unrecognized kinase controlling the maternal-to-zygotic transition (MZT) in teleosts 🐟 (2/13)
November 20, 2025 at 3:04 PM
Using our optimized CRISPR-RfxCas13d screening strategy, we identified Bckdk as a previously unrecognized kinase controlling the maternal-to-zygotic transition (MZT) in teleosts 🐟 (2/13)