Lucas Le Nagard
@lucasln.bsky.social
Postdoc in Edinburgh, Pilizota Lab | Biological Physics PhD | Interested in bacterial motility, soft matter, physiology, & fun experiments.
https://scholar.google.com/citations?user=i7JpZcUAAAAJ&hl=en&oi=ao
https://scholar.google.com/citations?user=i7JpZcUAAAAJ&hl=en&oi=ao
What are those three white blobs, and why do we spend hours at our microscopes recording movies like this? 🧐 Take a look at this short article on the 'bead assay' - a simple yet powerful technique that helps us, and many others, uncover the secrets of the bacterial flagellar motor!
March 12, 2025 at 2:59 PM
What are those three white blobs, and why do we spend hours at our microscopes recording movies like this? 🧐 Take a look at this short article on the 'bead assay' - a simple yet powerful technique that helps us, and many others, uncover the secrets of the bacterial flagellar motor!
The first direct result is that our protocols allow us to estimate experimentally the mechanical load under which the flagellar motors operate in free-swimming E. coli, which is hard to compute theoretically. We find the load to be equivalent to that of a 0.6 um diameter bead.
October 9, 2024 at 1:44 PM
The first direct result is that our protocols allow us to estimate experimentally the mechanical load under which the flagellar motors operate in free-swimming E. coli, which is hard to compute theoretically. We find the load to be equivalent to that of a 0.6 um diameter bead.
To confirm our findings, we studied cells that had both a small bead and a large bead. This way, both motors were driven by the exact same PMF. Again, small bead (low torque) motors remained sensitive to PMF in all conditions, while large bead speeds saturated at high PMF. (3/9)
October 9, 2024 at 1:42 PM
To confirm our findings, we studied cells that had both a small bead and a large bead. This way, both motors were driven by the exact same PMF. Again, small bead (low torque) motors remained sensitive to PMF in all conditions, while large bead speeds saturated at high PMF. (3/9)
New preprint! We find the relationship between the flagellar motor (FM) speed and the proton motive force (PMF) to be nonlinear in E. coli, limited by the maximum torque that a motor can supply. Surprisingly, this max. torque is reachable physiologically. (1/9)
biorxiv.org/cgi/content/...
biorxiv.org/cgi/content/...
October 9, 2024 at 1:40 PM
New preprint! We find the relationship between the flagellar motor (FM) speed and the proton motive force (PMF) to be nonlinear in E. coli, limited by the maximum torque that a motor can supply. Surprisingly, this max. torque is reachable physiologically. (1/9)
biorxiv.org/cgi/content/...
biorxiv.org/cgi/content/...