Light-Sheet Fluorescence Microscopy (LSFM) course
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lsfm-na.bsky.social
Light-Sheet Fluorescence Microscopy (LSFM) course
@lsfm-na.bsky.social
270 followers 250 following 33 posts
This is the US version of the long running course Pavel and Emmanuel have been running. We are just this side of the pond. Being held at MDIBL in Bar Harbor, Maine, June 2-6, 2025.
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lsfm-na.bsky.social
Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Apr 3
12 systems will be there, Bruker x3!, 3i, Leica, ASI, MBF, LiT, LifeCanvas x2!, and Miltenyi! Amazing cutting-edge technology, accessible instructors, and Bar Harbor! Please re-bluesky (what do you call it here:))!
mdibl.org MDI Biological Laboratory @mdibl.org · Apr 3
Have you been thinking of enrolling the MDI Bio Lab's #LightSheet Fluorescence Microscopy course?

Registration has been lowered to $1,000 — down from $3,500!
This is a great opportunity for researchers/students to enhance their knowledge & skills in cutting-edge #microscopy techniques. 🧪 🤝 🍎 👩‍🔬
Light Sheet Fluorescence Microscopy 2025
mdibl.org
Reposted by Light-Sheet Fluorescence Microscopy (LSFM) course
brandy-syglass.bsky.social
This was a great sample to check out -- who knew 100M year old amber would be so close to Ethyl Cinnamate in RI? Huge shout out to @brukercorporation.bsky.social for the fleet of lightsheets at @lsfm-na.bsky.social last week!
nesmicroscopy.bsky.social New England Society for Microscopy @nesmicroscopy.bsky.social · Jun 9
For #MicroscopyMonday, a 100M year-old 🦟 in amber! Lots of familiar #NESM faces up in Acadia for the #LSFM25 at the MDI @mdibiolab.bsky.social !

🔬: Bruker @brukercorporation.bsky.social, LCS SPIM
🟠: Courtesy the Sims Collection
🥼&🎥: Jan Frankowski @frankowskijc.bsky.social

@lsfm-na.bsky.social
June 9, 2025 at 7:30 PM
Reposted by Light-Sheet Fluorescence Microscopy (LSFM) course
nesmicroscopy.bsky.social
For #MicroscopyMonday, a 100M year-old 🦟 in amber! Lots of familiar #NESM faces up in Acadia for the #LSFM25 at the MDI @mdibiolab.bsky.social !

🔬: Bruker @brukercorporation.bsky.social, LCS SPIM
🟠: Courtesy the Sims Collection
🥼&🎥: Jan Frankowski @frankowskijc.bsky.social

@lsfm-na.bsky.social
June 9, 2025 at 4:03 PM
lsfm-na.bsky.social
And with that, #lsfm25 comes to a close! We want to thank everyone who helped make this possible - the faculty, course directors, TAs, vendors, and sponsors. But most importantly the many wonderful people at MDIBL. See you all next year!
June 7, 2025 at 1:40 PM
lsfm-na.bsky.social
Preparation for the final event of the course: a boat tour of Acadia!
June 6, 2025 at 9:57 PM
lsfm-na.bsky.social
Now that the students have had several days to learn sample preparation and image acquisition, we are wrapping up day five with a crash course called "Now what do you do with your data?" Led by @dpshepherd.bsky.social and Carsten Wolff.

#lsfm25 #microscopy #science #education
June 6, 2025 at 5:59 PM
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dpshepherd.bsky.social
Spending some time at #lsfm25 cleaning up code. It now has distributed GPU gradient-consensus RL decon (@jamesdmanton.bsky.social @andrewgyork.bsky.social) our fast orthogonal deskew, and stitching/fusion to ome-ngff v0.5. Example AO-OPM data on uncleared mouse brain slice github.com/QI2lab/opm-p...
Adaptive optics oblique plane microscopy of 100 um thick mouse brain slice with immunofluorescence.
June 4, 2025 at 6:56 PM
Reposted by Light-Sheet Fluorescence Microscopy (LSFM) course
brandy-syglass.bsky.social
Up in Acadia at the @mdibiolab.bsky.social for #LSFM25! Here's a cleared whole mouse head on the SmartSPIM, 3.6X objective for #3DThursday.

🔎 @jtakatoh.bsky.social, now at @stonybrooku.bsky.social
🔬 LifeCanvas Technologies, lifecanvastech.com/products/sma...
🥽 syGlass.io

@lsfm-na.bsky.social
June 5, 2025 at 12:54 PM
lsfm-na.bsky.social
We are so excited to start day four with a wonderful talk from Kara Cerveny!

#lsfm25 #science #microscopy #education
@mdibiolab.bsky.social
@kara-cerveny.bsky.social
June 5, 2025 at 1:01 PM
lsfm-na.bsky.social
It's day three and we are starting our morning off with an excellent intro to clearing by Doug Richardson.

#lsfm25 #microscopy #science #education
@mdibiolab.bsky.social
June 4, 2025 at 1:48 PM
lsfm-na.bsky.social
And it begins....
June 3, 2025 at 11:00 PM
lsfm-na.bsky.social
The calm before the storm indeed! In just a half an hour, this board will be bombarded by 25 students rushing to grab a spot on their instrument of choice. 11 systems. Only 9 labs. May the odds be ever in their favor...

#lsfm25 #microscopy #science #education
@mdibiolab.bsky.social
June 3, 2025 at 10:30 PM
lsfm-na.bsky.social
We are back from lunch with a talk from Raghav Chhetri telling us all about using light sheet microscopy for neuroscience imaging!

#lsfm25 #microscopy #science #education
@mdibiolab.bsky.social
@rchhmiscope.bsky.social
June 3, 2025 at 5:41 PM
lsfm-na.bsky.social
It's day two! We're starting off a day filled with science with a talk from Anna-Karin Gustavsson from Rice University.

#lsfm25 #microscopy #science #education

@mdibiolab.bsky.social
@riceuniversity.bsky.social
@gustavssonlab.bsky.social
June 3, 2025 at 1:11 PM
lsfm-na.bsky.social
Morning in Bar Harbor doesn’t suck.
June 3, 2025 at 10:26 AM
Reposted by Light-Sheet Fluorescence Microscopy (LSFM) course
ereynaud.bsky.social
Step 8: In @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Well day one done worth a sunset!
June 3, 2025 at 1:59 AM
Reposted by Light-Sheet Fluorescence Microscopy (LSFM) course
ereynaud.bsky.social
Step @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Went to collect some samples... Here is a pink tunic ate!
June 2, 2025 at 7:31 PM
lsfm-na.bsky.social
Reto Fiolka and OPM for the Keynote!
June 2, 2025 at 9:08 PM
lsfm-na.bsky.social
With the end of our first round of vendor talks behind us, we are excited to welcome Reto Fiolka as our keynote speaker this year.

#lsfm25 #microscopy #science #education
@mdibiolab.bsky.social
June 2, 2025 at 8:52 PM
lsfm-na.bsky.social
Holly Gibbs discussing the logistics of image processing and analysis
June 2, 2025 at 5:37 PM
lsfm-na.bsky.social
While we are on our lunch break, the students are enjoying some beautiful weather here at @mdibiolab.bsky.social.

#lsfm25 #microscopy #science #education
June 2, 2025 at 5:04 PM
lsfm-na.bsky.social
We now have Vera Morales from Nikon telling us all about objectives: the why, when, and how!

#lsfm25 #microscopy #science #education
June 2, 2025 at 3:02 PM
lsfm-na.bsky.social
Claudette St Croix from CBI kicks off LSFM 2025 at @mdibiolab.bsky.social
June 2, 2025 at 1:22 PM
Reposted by Light-Sheet Fluorescence Microscopy (LSFM) course
melcooperphd.bsky.social
@lsfm-na.bsky.social Is this heaven, or am I just currently surrounded by ELEVEN DIFFERENT LIGHT SHEET MICROSCOPES? So thrilled to be out here in Acadia chatting about microscopy!
June 2, 2025 at 1:01 PM
lsfm-na.bsky.social
LSFM 2025 has kicked off with an introduction from the one and only, @glaevsky.bsky.social, our course director.

#lsfm25 #microscopy #science #education
June 2, 2025 at 12:53 PM
lsfm-na.bsky.social
Calm before the storm…
June 1, 2025 at 8:50 PM