Lew Lab @ WashU
@lewlab.com
Playing with #singlemolecules, bending light, and algorithms to see really small things. Led by Matthew Lew, Associate Professor @WashUESE @WashUengineers @WashU #superresolution #microscopy #SMLM #computationalimaging
Now in print: our study using single #fluorogenic molecules to probe the network architecture within #biomolecular #condensates with @rohitpappu68.bsky.social @washuengineers.bsky.social . Teaser video below. Read the full article at doi.org/10.1038/s415... #singlemolecule
May 30, 2025 at 4:16 AM
Now in print: our study using single #fluorogenic molecules to probe the network architecture within #biomolecular #condensates with @rohitpappu68.bsky.social @washuengineers.bsky.social . Teaser video below. Read the full article at doi.org/10.1038/s415... #singlemolecule
- Matt will speak at 8:00 AM on “ #SingleMolecule #Orientation #Localization #Microscopy: New Approaches and Applications” (NM1C.1).
- Yiyang will speak at 9:15 AM on “Combining Excitation and Emission Modulation Resolves the Angular Separation between a Pair of #DipoleEmitters” (NM1C.4).
- Yiyang will speak at 9:15 AM on “Combining Excitation and Emission Modulation Resolves the Angular Separation between a Pair of #DipoleEmitters” (NM1C.4).
April 20, 2025 at 2:17 PM
- Matt will speak at 8:00 AM on “ #SingleMolecule #Orientation #Localization #Microscopy: New Approaches and Applications” (NM1C.1).
- Yiyang will speak at 9:15 AM on “Combining Excitation and Emission Modulation Resolves the Angular Separation between a Pair of #DipoleEmitters” (NM1C.4).
- Yiyang will speak at 9:15 AM on “Combining Excitation and Emission Modulation Resolves the Angular Separation between a Pair of #DipoleEmitters” (NM1C.4).
Read our article here: doi.org/10.1103/Phys...
Resolving the Orientations of and Angular Separation Between a Pair of Dipole Emitters
We prove that it is impossible to distinguish two spatially coinciding fluorescent molecules from a single rotating molecule using polarization-sensitive imaging, even if one modulates the polarizatio...
doi.org
March 21, 2025 at 4:15 AM
Read our article here: doi.org/10.1103/Phys...
Reposted by Lew Lab @ WashU
"Single-flurogen" is key, allows them to view condensate hubs that are normally a needle in a haystack to find. The use of a single chemical probe means they don't get conflicting signals. 🧪
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Single-fluorogen imaging reveals distinct environmental and structural features of biomolecular condensates - Nature Physics
Experiments confirm the prediction that fluid-like biomolecular condensates are defined by spatially inhomogeneous organization of the underlying molecules.
www.nature.com
March 14, 2025 at 2:56 PM
"Single-flurogen" is key, allows them to view condensate hubs that are normally a needle in a haystack to find. The use of a single chemical probe means they don't get conflicting signals. 🧪
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Thanks @leahshaffer.bsky.social for your wonderful coverage of our collaborative work with @rohitpappu68.bsky.social ! @washuengineers.bsky.social #singlemolecule
March 14, 2025 at 3:12 PM
Thanks @leahshaffer.bsky.social for your wonderful coverage of our collaborative work with @rohitpappu68.bsky.social ! @washuengineers.bsky.social #singlemolecule
It's a pleasure to see this collaborative work with the Pappu lab @rohitpappu68.bsky.social in print! @washu.bsky.social @washuengineers.bsky.social
March 14, 2025 at 2:38 PM
It's a pleasure to see this collaborative work with the Pappu lab @rohitpappu68.bsky.social in print! @washu.bsky.social @washuengineers.bsky.social
Perhaps W. E. is getting royalties? @samjlord.bsky.social @juliebiteenlab.bsky.social @weisslab.bsky.social @mauriceylee.bsky.social
December 27, 2024 at 7:17 AM
Perhaps W. E. is getting royalties? @samjlord.bsky.social @juliebiteenlab.bsky.social @weisslab.bsky.social @mauriceylee.bsky.social
The bottom image is from our multi-view microscope work, showing amyloid aggregate infiltration of a spherical lipid bilayer: doi.org/10.1038/s415...
Six-dimensional single-molecule imaging with isotropic resolution using a multi-view reflector microscope - Nature Photonics
A multi-view reflector microscope based on polarization modulation and pupil splitting enables single-molecule orientation-localization microscopy with precisions of 10.9 nm and 2.0°.
doi.org
December 26, 2024 at 4:57 PM
The bottom image is from our multi-view microscope work, showing amyloid aggregate infiltration of a spherical lipid bilayer: doi.org/10.1038/s415...
Top-right image is from Ezra and the Lee lab's beautiful POLCAM work of actin fiber networks. The POLCAM software is much easier to use than full PSF fitting: doi.org/10.1038/s415...
POLCAM: instant molecular orientation microscopy for the life sciences - Nature Methods
Combining localization and polarization microscopy can yield detailed insights into subcellular structures. POLCAM uses a polarization camera and wide-field microscopy for rapid measurement of super-r...
doi.org
December 26, 2024 at 4:56 PM
Top-right image is from Ezra and the Lee lab's beautiful POLCAM work of actin fiber networks. The POLCAM software is much easier to use than full PSF fitting: doi.org/10.1038/s415...